This allows an equal concentration of protein from each sample to be load into the wells later on when the gel electrophoresis is run. The Coomassie dye is brownish/red in color but turns blue when mixed with proteins. The blue color can be measured in a spectrophotometer at 595nm. A standard curve was generated using known amounts of bovine serum albumin (BSA). Four microfuge tubes were set
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Introduction What is Protein? There are more than 300 amino acids in the nature. However‚ only 20 amino acids are joined by each other with peptide bonds in different sequences and numbers. These sequences are formed by helping of genetic information which is encoded in genes. So‚ each protein molecule has its own amino acid sequence. Proteins are most abundant long chain macromolecules in all cells and they serve as structure and function matter in cells of all living organisms such as hormones
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Throughout history many people have been overshadowed by someone that is seen as more accomplished. The ones that are overshadowed are sometimes forgotten about. However‚ this does not make them less important. Sir Henry Morton Stanley is one of these people who was overshadowed. Although he may be seen as inferior to other people‚ he did a lot in his lifetime. On January 28‚ 1841‚ Sir Henry Morton Stanley‚ original name John Rowlands‚ Congolese name is Bula Matari‚ “Breaker of Rocks”‚ was born
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Question 5: The chromophore in this assay is Coomassie Brilliant Blue dye. Question 6: It is important to set up a blank to separate the solute (saline) from the protein (stock). By subtracting the absorbance of the blank (which has no protein present) from the original absorbance the absorbance of the protein at each concentration will remain. Question 7: The Lowry method relies on two different reactions. The first is the formation of a copper ion complex with amide bonds‚ forming reduced
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Abstract The experiment‚ entitled Extraction and Characterization of Proteins‚ aims to isolate casein from milk and albumin from egg; to explain the methods employed for protein extraction; to apply spectrophotometric methods in characterizing and quantifying extracted casein and albumin. The experiment was divided into 2 parts; the extraction of Albumin from egg and the determination of protein concentration via the Warburg-Christian method and Bradford Assay method. In the first part‚ egg
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Erythrocyte: Structure & Metabolism หัวข้อบรรยาย 1. Red cell membrane 1. Membrane lipid 2. Membrane skeleton 3. Peripheral proteins 4. Integral proteins 2. RBC metabolism 1. Glycolytic (Embden-Meyerhof) pathway 2. Hexose monophosphate shunt 3. Rapoport-Luebering pathway 2.4 Methemoglobin reductase pathwa 3. Hemoglobin 3.1 Hemoglobin structure 3.2 Hemoglobin synthesis
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Expression and Purification of Human Platelet-Derived Growth Factor Beta (PDGF-B) Rachel C. Hermina-Stewart* and Elsie I. Parés-Matos† *Industrial Biotechnology Program‚ †Department of Chemistry University of Puerto Rico-Mayagüez Campus rachel.hermina@upr.edu December 14‚ 2009 ABSTRACT. Platelet-Derived Growth Factor (PDGF) is one of many proteins that regulate cellular growth and division. The PDGF family consists mainly of five different isoforms called as PDGF-A‚ PDGF-B‚ PDGF-C and PDGF-D. Three
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young slave girl called Abina Mansah lived in the British Gold Coast Colony (now Ghana) in 1876. She was born in Asante/Ashanti. Abina had been captured in Asante area‚ and enslaved in two houses in Asante‚ the first one was in the capital city of Coomassie at Eddoo Buffo’s home and the second one in Asante territory of Adansi20. Abina’s life events did not happen by accident. Everyone’s life shaped by things or people around us for exmaple family‚ friends‚ and culture. Sometimes these things around
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EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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Introduction: The purpose of this experiment is to see the anti-cow antibody bind to cow serum only‚ and we expect to see the anti-cow antibody bind to the spot that had the cow serum. The system we use in this experiment is the serum from Cow‚ Horse‚ Goat‚ Sheep‚ and Donkey‚ Chicken. In order to able to detect and analyze proteins based on their ability to bind to a specific antibody‚ the SDS-PAGE and Western Blot was performed. SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a very common
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