The environmental factors that effected the rate of enzyme reactions were the enzyme concentration‚ pH‚ and temperature. These environmental factors help enzymes break down the poisonous chemicals into harmless substance. When we tested the liver with 2ml of hydrogen peroxide for a normal reaction it showed that it was exothermic. We added more hydrogen peroxide and the reaction rate of the liver was 3. We learned that the catalase is reusable because the liver reacted both times when we put in
Premium Oxygen Enzyme Hydrogen peroxide
If pH > pI‚ then the protein will have a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH
Premium Acid Acid dissociation constant Base
Introduction DNA‚ or deoxyribonucleic acid‚ is a double stranded helical structure used to store genetic information in cellular organisms. DNA usually consists of two strands made up of nucleotides‚ each with a backbone of repeating units of phosphate groups and the sugar‚ deoxyribose‚ bonded by phosphodiester linkages. Since the deoxyribose has a specific orientation‚ DNA molecules have directionality so that DNA sequences are read 5’ to 3’. The 5’ end of DNA is characterized by the deoxyribose
Premium DNA Gene Genetics
1: INTRODUCTION When studying the function of catalysts in reactions during the kinetics unit‚ I was eager to know more about the position of enzymes‚ which function as biological catalysts in biological systems. After doing some further research‚ I found that catalase‚ an enzyme‚ which is found in nearly all living organisms such as animals‚ catalyses the decomposition of hydrogen peroxide (H2O2) in the blood. H2O2 is produced by reactions in the white blood cells in our body to fight against diseases
Premium Oxygen Hydrogen peroxide Chemical reaction
Introduction: A chemical reaction involves the breaking and forming of bonds in order to create the necessary energy required to cause movement. Each reaction is catalyzed (an increase in rate because of a present catalyst) by a specific enzyme. Enzymes are able to denature proteins‚ meaning that a protein loses its original shape by uncoiling‚ giving it a random‚ unstructured shape. The pineapple plant contains bromelain which‚ because of its unique characteristics‚ keeps gelatin from thickening
Premium Enzyme Chemical reaction Metabolism
Experiment three was divided into three parts; the preparation of the solutions‚ the reaction between Hydrochloric Acid and Ammonium Hydroxide (part b)‚ and the formation of Magnesium Oxide (part c). 200 mL of deionized water were added to a beaker followed by the addition of 100 mL 6 M HCl‚ which reacted to make 300 mL of a 2 M HCl solution used for Part B. Next‚ 50 mL of deionized water were added to a separate beaker and then 100 mL 3 M NaOH were added to the beaker to form 150 mL of a 2 M NaOH
Premium Chemistry Water Sodium hydroxide
experiment‚ we were trying to see whether copper‚ after a chain of chemical reactions‚ will revert back to its elemental form. Procedure: First‚ I added nitric acid with copper in a beaker‚ which turned into a copper nitrate‚ a blue-green solution. Afterwards‚ I added sodium hydroxide‚ and my solution colored to a dark blue solution called copper hydroxide. I heated the solution to evaporate the water and I got a brownish-blackish solid called copper oxide. Once the solid appeared‚ I poured
Premium Chemistry Sulfuric acid Copper
All chemical reactions are processed through energy exchanges. Chemical reactions either absorb energy or release energy into their surroundings. In thermodynamics‚ endothermic reactions absorb energy where exothermic reactions release energy. In this experiment‚ we will be observing the chemical reactions that occur when a specific liquid is combined with a specific solid. We will measure the temperature of the liquid before the solid is added. Then we will measure the temperature of the liquid
Premium Chemical reaction Temperature Chemistry
Demonstration of the Rates of Reaction Between Sulphuric Acid (25mL ±0.5mL) and Magnesium (0.02g ±0.01g) Changing Due to Different Surface Areas By Chania Baldwin Introduction: When sulphuric acid and magnesium are added together‚ magnesium sulphate and hydrogen gas is created. To create such a reaction the atoms must collide with a sufficient amount of energy. Every reaction requires a different amount of energy to create the reaction‚ which is called the activation energy; when there is not enough
Premium Chemical reaction Chemical kinetics Surface area
by Georg Wittig in 1954‚ the Wittig reaction is a robust organic synthesis method for preparing stereospecific alkenes. In general‚ Wittig reactions involve an aldehyde or ketone and a Wittig reagent (triphenylphosphonium ylide) and result in the formation of an alkene product and triphenylphosphine oxide (side product). Stereospecific alkene products can be synthesized by adjusting the reaction reagents and conditions. In the 60 years since the Wittig reaction was discovered‚ many articles have
Premium Alkene Aldehyde Chemical reaction