Enzymes Reactions to Changes in Substrate and Inhibitors Benjamin J. Mora Coronado University of Texas Rio Grande Valley at Edinburgh Abstract Purpose for the experiments was to test the enzymes in various scenarios and see how changing this would affect the rate of reaction. The enzyme source used in the experiments was Turnip Extract. Concentrations of Turnip extract for activity 1 where o.5ml‚ 1.0ml‚ and 2.0 ml as for the rest of the activities 2 Through 4 stayed at a consistent concentration
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Temperature is a measure of kinetic energy. As this movement increases‚ collision rate and intensity‚ and therefore reaction rates‚ increase. This experiment was conducted to determine if there is a minimum temperature that increase kinetic energy and denature enzymes to slow enzymatic reactions or fail to catalyze them. The experimental results indicate an increase in temperature will increase reaction rates until proteins denature. Introduction Enzymes combine with reactant molecules (substrate) and
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There are many types of chemical reactions‚ five general ones of which I will be describing. Chemical changes are a result of chemical reactions. All chemical reactions involve a change in substances and a change in energy. Neither matter nor energy is created or destroyed in a chemical reaction‚ only changed. There are so many chemical reactions that it is helpful to classify them into 5 general types‚ which include the following: synthesis‚ combustion‚ decomposition‚ single-replacement‚ and double-replacement
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Reaction Order and Rate Law Name Data‚ Calculations‚ and Questions A. Calculate the initial and final concentrations as needed to complete Tables 1 and 2. Data Table 1: Varying the Concentration of 1.0 M HCl | | | | |Concentrations | | |# Drops |# Drops |# Drops |Initial
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Determination of Rate and Order of a Reaction Results This experiment used a spectrometer to find the wavelength with maximum absorbance in a green food coloring solution. For this particular solution the wavelength was 629.7 nm. The system was then calibrated to that and was set to measure the food coloring and bleach solution. The measured visible light absorbance of the mixed solution was collected over a time of 200 seconds and eight points were then selected and placed into the Absorbance
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Introduction The purpose of this lab is to be able to observe the reaction rates of different chemical substances‚ by looking at which substance is the fastest reactant. This is what chemical kinetics is. Reaction rate is the change in the concentration of a reactant or product in a chemical reaction per unit time. In this lab the requirement was to be able to calculate each Average reaction rate which is the change in reactant or product concentration at a given time interval. Some equations that
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Megan Wierzbowski Organic II Laboratory Wed. 11-1:50 Title: Grignard Reaction with a Ketone: Triphenylmethanol Part 1 Objective: The goal of this experiment is to learn to make Grignard reagents. The reactions of the Grignard reagents with ketones form tertiary alcohols. These reagents are highly air- and moisture-sensitive materials. We will observe the formation of the Grignard reagents‚ which magnesium metal is transformed into organometallic salts. Experimental Procedure:
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Title: Effect of a Catalyst on Reaction Rate II. Introduction: The basis of the experiment is the enzyme; an enzyme is a biological molecule that acts as a highly selective catalyst. By combining with a substrate‚ an enzyme is able to create a new product that helps the body function. (Ex. Lactose/Lactase) A substrate is a molecule an enzyme acts upon‚ the two combine at an area called an active site. This active site allows induced fit which allows the reaction to occur and a new product to be
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much of the solids may cause chemical splattering or splashing. Wear chemical splash goggles‚ chemical-resistant gloves‚ and a chemical-resistant apron. Wash your hands with soap and water before leaving the lab. Procedure Part A: The Overall Reaction (refer to lab handout) Procedure Part B: Controlled Experiments! 1) Mix only the calcium chloride and red phenol‚ and observe any changes that occur. 2) Mix only the sodium bicarbonate and red phenol‚ and observe any changes that occur. 3) Mix
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The E2 reaction performed began by dehydrohalogenating the 3-chloro-3‚ 7-dimethyloctane with potassium hydroxide in aqueous ethanol. The 3-chloro-3‚ 7-dimethyloctane will lose a hydrogen from three different carbons that cause the formation of three constitutional isomers. If the hydroxide ion attacks a hydrogen on the 3-methyl carbon‚ then 2-ethyl-6-methyl-1-heptene will be the product. If a hydrogen on the carbon-2 was attacked‚ then the product would be 3‚ 7-dimethyl-2-octene. If a hydrogen was
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