buffer was made. We began this experiment by measuring seven constant amounts of 1ml of 0.1% catechol using a 1 mL pipet into each seven cuvettes. The catechol is our substrate solution. Next‚ different amounts of phosphate buffer ph.6 of 0.5ml‚ 1.0ml‚ 1.5ml‚ 2.0 ml 2.5ml‚ 3.0ml and 4.0ml were added to each of the solution of the 0.1% catechol. The purpose of adding phosphate buffer pH6 is to maintain the particular ph6 for the polyphenol-oxidase (enzyme)‚
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was first determined. Acetanilide was produced by acetylation of aniline with acetic anhydride. The crude acetanilide was dissolved in a solvent in a heating water bath. The hot solution was filtered and the filtrate‚ cooled slowly in an ice bath as crystals started forming out. As the compound crystallizes from the solution‚ molecules of other compounds were excluded from the crystals to obtain pure acetanilide. INTRODUCTION Recrystallization‚ referred to as the second crystallization‚ is a method
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Effects of temperature manipulation and solution treatment on the Beta vulgaris craca plant cell membrane and the change of the concentration of betacyanin when placed under these various stresses Introduction: The Beta vulgaris craca plant‚ commonly referred to as the beet root contains a pigment‚ red in colour‚ called betacyanin. The betacyanin’s containment within the cells of the beet root cell relies on the stability of the plant’s membrane structure. The manipulation of the cell’s membranes
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standards. Further‚ I learned more about the molarity of aqueous solutions‚ and how that quantity‚ along with the volume of the solution‚ can be manipulated to find the exact number of moles in a given volume. In this case‚ experimenters were given aqueous solutions of NaOH and CaCl2 in known molarities and then had them react with one another to yield a precipitate of Ca(OH)2. The precipitate was filtered out of the remaining aqueous solution of stoichiometry. In our case‚ all four tests yielded more
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are avoiding put-downs‚ connecting solutions‚ listening for data‚ and summarizing ideas. Being able to overcome these challenges will bring about successful group discussions. Challenge 1: Avoiding put-downs are extremely important. Put-downs occur when members of the group ridicule the ideas that are presented by other members. When this happens‚ it can slow down the productivity of the group‚ and can stop other members for voicing their own ideas or solutions. One way to solve this problem is
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cups with two hundred fifty milliliters of water. Then‚ mixing teaspoons of salt in water one at a time‚ until you’re able to submerge a raw egg in the solution and have it float up. My hypothesis is‚ salt does infact change water’s density‚ and if the egg floats it’s proof of density change in the water‚ because it must be less dense than the solution in order to float. The procedure is as follows‚ I filled two cups with two hundred fifty milliliters of water and mixed four teaspoons of salt in one
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concentration gradient the diffusion that did occur happened a lot faster and diffused more efficiently compared to no iodine solution and just water. This is due to the molecular collisions speeding up the experiment in the time period given because of the Soule molecules found within the given volume. This experiment can also refer to hypo‚ hyper and isotonic due to the two solutions being separated by the dialysis tubing (which acts as a semipermeable membrane) and diffusing from
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solute particles is greater than the other solution. Core B was isotonic meaning that it had an equal balance throughout. When two environments are isotonic it means that the total molar concentration of dissolved solutes is the same in both of them (equal). When cells are in an isotonic solution‚ movement of water out of the cell is exactly balanced by movement of water into the cell. Core C was the most flexible which made it be hypotonic. A hypotonic solution expresses that the total concentration
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objective of this lab was to recreate the color profile of a given solution. In this case‚ the solution was Powerade. The final solution should match the absorbance values at the peak wavelengths (420nm and 628nm) in Powerade. This lab was done using deionized water‚ FD&C Blue #1‚ FD&C Yellow #5‚ FD&C Red #40‚ and a spectrometer. To obtain the correct color profile‚ FD&C Blue #1 and FD&C Yellow #5 were utilized in the sample solutions. The experiment was conducted over two days; the first day was reserved
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5.5 Candy Chromatography Background Information: Paper Chromatography is a separation tool in which pigment is put on a paper made of cellulose and water‚ and placed in a solvent‚ in this case isopropyl alcohol. Due to capillary action‚ the solvent crawls up the paper‚ separating the pigments. This technique is used to identify components of a mixture‚ even unknown ones‚ and can be used to isolate components into pure samples. Real world uses of this technique includes identifying certain biomolecules
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