CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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Paper chromatography is an important separation technique that depends upon differences in how strongly the dyes are adsorbed onto the paper (stationary phase) and how soluble the dyes are in the developing solvent (mobile phase). In paper chromatography‚ a small amount of the mixture to be separated is placed close to the edge of a piece of paper. The edge of the paper is then immersed in a developing solution. As the developing solution ascends up the paper by capillary action‚ the. components
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Bleach Oxidation of 9-Hydroxyfluorene The purpose of this experiment was to oxidize an alcohol (9-hydroxyfluorene) to a ketone (9-fluorenone) using aqueous sodium hypochlorite (bleach) as the oxidizing agent‚ while introducing techniques used in microscale experiments. Reaction: Results 1. Recrystallized Product Yield Product yield = (actual yield/theoretical yield) x 100% 3mL 9-hydroxyfluorene x (1mL/1000mL) x (0.09 mol/L) = 2.7 x 10-4 moles 0.05g 9-fluorenone / (180.20g/mol) = 2.77 x 10-4
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Name ________________________12345_______________________ Date ____23.8.2013___________ Block _______ Training Lab: Paper Chromatography I. Background Information: When working in a lab‚ scientists often need to identify different molecules that are present in a sample they are studying. There are many ways to identify unknown molecules/chemicals in a sample. The method you will be using today is called Paper Chromatography and consists of 2 steps. First‚ you will separate the unknown chemicals
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Unit2 Assignment2 Vulnerability of a Cryptosystem The vulnerability that has been discovered has a primary affect to a cryptosystem and a secondary affect to a cryptosystem. The vulnerability in question is a weakness in the MD5 algorithm that would allow for collisions in output. As a result‚ attackers can generate cryptographic tokens or other data that illegitimately appear to be authentic. Now the secondary affect is that the MD5 hashes may allow for certificate spoofing on a Cisco ASA system
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CHROMATOGRAPHY _TOPIC_ : 7.2 _RESEARCH QUESTION:_ What is chromatography? How many pigments are there in a plant? How the different pigments in a plant can be separated? _APPARATUS_ : Please refer to the handout _MATERIAL_ : Please refer to the handout _METHOD_ : Please refer to the handout _DATA COLLECTION_: Coloured band Pigment front distance (± 0.05cm) Solvent front distance (± 0.05cm) Green 11.7 13.0 Yellow 12.5 13.0 _DATA PROCESSING_: Coloured band Pigment
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Separation Methods Submitted By: Tiffany Lam Partner’s Name: Morgan Silveira Date of Submission: September 25‚ 2013 Lab Section: CHE110L*Z Purpose The purpose of this experiment is to use two common laboratory methods to see how mixtures can be separated based on physical properties. Paper chromatography will be used to separate the dye colors from M&M candy to see which colors contain yellow #5. Gravimetric separation will be used to separate salt and sand from
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Separation Of A Dye Mixture Using Chromatography ********* AP Chemistry (3rd Block) Fauquier High School 8/ 31/ 13 ABSTRACT: The Rf values of seven known color dyes and three unknown samples were observed in order to compare results and discover the hidden identities of the three samples. Three solvents‚ H2O‚ Isopropyl alcohol‚ and a 2% NaCl solution‚ were introduced to the dotted chromatography strips to show three different ratios for each dye on
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Assignment: Work Group Scenarios For this exercise‚ let us assume that the processes required have been completed so that we may now add users and then create a work-group in the ACMExx.COM Domain. To clarify the required steps before we would be prepared to add users lets review the steps in a broad sense: Create a “Shared” Folder on the desktop Install and configure a WINS Install and Configure a DHCP Server Create a SCOPE and determine how the addressing of users will be handled Create
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Exercise 1: Encrypt the plaintext below using the Caesar cipher: THE CAESAR CIPHER IS A TYPICAL EXAMPLE OF A SUBSTITUTION CRYPTOSYSTEM. t 19 22 w a 0 n 13 b 1 o 14 c 2 p 15 d 3 q 16 e 4 r 17 f 5 s 18 g 6 t 19 h 7 u 20 i 8 v 21 j 9 w 22 k 10 x 23 l 11 y 24 m 12 z 25 Encryption formula: f(x)= x+k (mod26) Character to be shifted to the right by k; x is the characterʼs
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