helped me prepare for the final lab‚ identification of an unknown bacterium. For the final lab‚ I received the organism “6A”. To start identifying this organism‚ I did a gram-stain to identify if the organism is gram positive or negative. I created two slides to ensure that I did the stain correctly with similar results. The gram-stain consists of creating a smear from the given plate and fixing it to the plate with methanol‚ then‚ dying the smear with crystal violet for
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and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink Microscope Clothes pin Bunsen burner Methods: 1. Obtain your slide and draw two circles where your bacteria will go. 2. Connect you Bunsen burner and run your inoculation loop through
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means that the point of view or statement put forward is illogical‚ inadequate‚ not sound or can be shown to be wrong. Hydrates are inorganic salts that hold water. They contain a specific number of strongly bonded water molecules as part of their crystal structure. Such water molecules are called water(s) of hydration‚ or water(s) of crystallization. The formula of a hydrate consists of the formula of the anhydrous (without water) compound followed by a dot‚ then the specific number of water molecules
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bacteria are not gram positive‚ but this lab will examine only this kind because of their influence in human health. Staphylococcus Aureus is a gram-positive coccus bacterium found in humans. It is frequently found on the mucus membrane in the nasal cavity and on the skin. It also infects many people in the hospital‚ also called a nosocomial infection. S. aureus has a large association with clinically significant infections and
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Endospore and Capsule Cell Staining Allison Lui Partner: Mary Chagin BIOL-235 W07 Dr. Runco 2/10/15 Introduction The purpose of this lab is to learn what endospore and capsules are‚ and how to identify them under a microscope using capsule and endospore staining methods. Capsules are found only in select bacteria‚ and serve a protective purpose. Made out of sugars and proteins‚ they are antiphagocytic‚ which prevents other cells to engulf the bacteria through phagocytosis. It
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with the information gathered‚ it was identified that the unknown sample was Streptococcus salivarius. Introduction: In this lab‚ the exploration of an unknown gram positive bacteria was conducted. What is known about gram positive microorganisms is that their cell walls are composed of a thick peptidoglycan layer‚ which retains the purple color of crystal violet during a Gram stain.
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Worley February 21‚ 2012 Mitchell Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining‚ Capsule Staining‚ Endospore Staining‚ and Acid Fast staining. One of the most significant method of staining is
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deionized water. Next‚ for Water of Hydration section‚ obtain a few crystals of hydrated copper(II) sulfate‚ describe their appearance on the data sheet. Then place the crystals into an evaporating dish‚ place a watch glass over the top of the dish and heat to drive off the water. Move the
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Unknown Lab Report Unknown Organism #6 Ann Le (Phuoc) May 6‚ 2010 Dr. Carrington Microbiology Lab- MW 12:50 Le 1 I. Introduction My unknown organism #6 is Morganella morganii‚ which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans‚ mammals‚ and reptiles as a normal flora. (3‚ 5) This bacterium Morganella morganii‚ was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide
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Abstract Through three different experiments we were to find the quality of NaCl as a deicer. These experiments included testing the freezing point depression of NaCl in solution at two different concentrations‚ testing for the H of an aqueous NaCl solution‚ and combining NaCl with MgCl2 in an aqueous solution to test for freezing point depression. Through these various experiments we found NaCl to be the best deicer for our region due to its proficient freezing point depression and its cost
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