EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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Irrational use of water resources - Water is the most important element of any creature’s life. A person can survive weeks without any food‚ but none can survive 3 days without any water. People waste water in their homes without even realizing it. We have become so accustomed to have a 24 hour supply of water to meet all of our needs from cooking‚ to cleaning‚ to drinking‚ that we sometimes forget that we do not have an infinite supply of water. Everyday water is wasted through leaky taps
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A Message to All Earthlings from Your Friend Tudor the Just People of Earth‚ warmest and friendliest greetings! I am Tudor the Just and I come from Neptune‚ as you know if you have been watching the news. By the way‚ I want to congratulate all of your TV networks on their coverage of my landing. They did a galaxy-class job. It made me think about how much your reporters deserve the chance to cover real news—like the explosion in Cluster H356pi or the rebellion on Altair 7. The truth is‚ all
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Victor Frankenstein says‚ “I pursued nature to her hiding-places...which made me the neglect the scenes around me caused me also to forget those friends...whom I had not seen for long time.” (33). This isolation only seems to get worse when the Creature comes to life and fear
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Hot Dog A Global History Bruce Kraig the edible series Edible Series Editor: Andrew F. Smith is a revolutionary new series of books dedicated to food and drink that explores the rich history of cuisine. Each book reveals the global history and culture of one type of food or beverage. Already published Pancake Ken Albala Pizza Carol Helstosky Hamburger Andrew F. Smith Spices Fred Czarra Pie Janet Clarkson Forthcoming Bread William Rubel Cake Nicola Humble Caviar
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Some people thinks being alone may shorten lives. But contrary to all beliefs isolation actually helps people make it through the day. Whether it is for an hour‚ 3 hours or even 5 hours. Isolation has its fair share of advantages. Being around people constantly can sometimes take a toll on people ‚ so being alone for a while is well needed in today’s society. There’s so much going on today. Terrible things are happening everyday‚ it’s hard to not want to just get away from people and the social
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Wuthering Heights‚ by Emily Bronte‚ is set in the detached Yorkshire moors during the early nineteenth century and depicts the lives of two contrasting families. Because Wuthering Heights was written during the Romanticism movement‚ many characteristics of the movement are reflected by the novel. The characters’ reasons for becoming isolated are universal and can be connected to situations found in modern music. Bronte reveals universal aspects of the human condition by highlighting the manner in
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Report The Goal Hong Kong is a world-renowned economic metropolis‚ and famous for its efficiency and well-serviced community. However‚ it has over 7 million inhabitants living in an area of 1‚000 square kilometers‚ and the population is still growing. Projections established by China’s Census‚ and their statistics show the population could be increased by another 2 million over the next 30 years[overpopulation.com‚ 2013]. The majority of the increase‚ 93%‚ is caused by immigrants[overpopulation
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In the novel‚ Ender’s Game written in 1977 by Orson Scott Card a futuristic story reveals the anguish during the struggle for survival. In chapter four to five‚ Ender faces the isolation‚ which is caused by Graff on purpose‚ however he has learned a lesson from Graff that in this world the one he can really rely on is himself. Later on he unfolds his progress that he modifies the isolated situation. As a result‚ Ender’s developing self-reliance is setting up his mind to be strong enough to face the
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In the first reading‚ “Extreme Isolation” by Kingsley Davis‚ the author discusses the findings of the research performed on the infant Anna. Anna was unlike any other child at her age. During the first few months of her life‚ she was passed around from home to home‚ unable to return to her mother. She had caught many diseases along the way‚ which allowed her chances for a home to become slim. She was taken back to her mother and grandfather and was kept in isolation for many years until she was found
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