The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
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Chemical Reactions Lab Introduction Four different types of chemical reactions were observed in this lab. Synthesis reactions occur when two different elements or compounds form a more complex compound as shown by equation (1). Single replacement occurs when one element replaces another to form a new compound as shown in equation (2). Double replacement occurs when two different atoms in different compounds trade places with one another to form two new compounds as shown in equation (3).Combustion
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It was originally hypothesised that the reaction time would decrease with higher concentrations of the reactants‚ potassium iodate and sodium bisulfite as collision theory stated that more molecules present in a given volume meant a greater chance they would collide. Furthermore‚ the time was expected to decrease with higher temperatures as it influenced the reactant particles by raising their kinetic energy. However‚ the data collected from this experiment only partially supported this hypothesis
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the solid copper(II) nitrate is used to dissolve in a test tube filled with half distilled water. The solid copper(II) nitrate ionize in water to form copper(II) ions and nitrate ion. Hence‚ a blue solution is formed due to the blue copper(II) ion present in the solution. When excess solid is added‚ the solution will become saturated and do not allow any solid to dissolve‚ so excess solid will remain in the solution. This is shows that the equilibrium between solid and aqueous copper(II) nitrate
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This experiment was performed to demonstrate a cycle of chemical reactions involving copper. This lab will start with copper as a reactant in the first reaction through a series of five chemical forms of aqueous phase reactions and ending to calculate the percentage of recovered solid copper as a product in the last reaction of the experiment. The experiment resulted in a percent recovery from the cycle of copper reactions of an increase to 139%. . Introduction: The purpose of this experiment is
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Observing a Single Chemical Reaction Qualitative vs. Quantitative Observations Abstract: The purpose of this experiment is to help you sharpen your observational skills. The aluminum foil was found to rust in the water containing the dissolved Copper Chloride (CuCl₂) crystals. The results of the experiments were determined through close examination and observation of both qualitative and quantitative elements of the changes that occurred when a blue crystal‚ Copper Chloride‚ reacted with aluminum
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Constant of an Unknown Ester Hydrolysis Reaction Abstract The experiments to follow determined that the equilibrium concentrations of the reaction: ester + water ↔ alcohol + acid‚ are equal to 0.0363 moles of ester‚ 0.2852 moles of water‚ and 0.0268 moles each of alcohol and acid. Using this information the equilibrium constant was determined to be 0.06938. 1. Introduction In this lab the equilibrium constant‚ Kc‚ for the acid catalyzed reaction between an unknown ester and water
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Chemical Reactions Lab Objectives: 1. To examine a variety of reactions including precipitation‚ acid-base‚ gas forming‚ and oxidation-reduction reactions. 2. To identify the products formed in these reactions and summarize the chemical changes in terms of balanced chemical equations and net ionic equations. 3. To identify the species being oxidized and reduced in oxidation-reduction reactions and determine which species is the oxidizing agent and the reducing agent. Chemical equations represent
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Erin Bolton Chemistry Lab Report April 29‚ 2015 Lab: Reaction Rates Introduction: In this experiment we studied the reaction of potassium persulfate‚ K2S2O8‚ with potassium iodide‚ KI. All chemical reactions have an energy barrier to overcome before the reaction will proceed. We will record data based on the concentration‚ temperature and catalyst for each experiment. Once this has been completed it will be graphed. Procedure: Due to the chemicals being used having hazard gloves are used
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experiment the objective was to perform a Diels-Alder reaction using cyclopentadiene and maleic anhydride to synthesize the product‚ cis-Norbornene-5‚6-endo-dicarboxylic anhydride. The Diels-Alder reaction is one of the most important reactions in organic chemistry and was first investigated by Otto Diels and Kurt Alder in Germany. It is a [4+2] concerted cycloaddition reaction which involves a diene and a dienophile. The Diel-Alder reaction are mainly used for creating new carbon-carbon bonds‚ mostly
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