Philippine Normal University- Mindanao Campus‚ Prosperidad‚ Agusan del Sur‚ Philippines Abstract The present study was conducted to determine the lethal concentrations of the different parts of Dioscorea hispida through Brine Shrimp Lethality Assay. Cytotoxicity was evaluated in terms of lethal concentration. Each part of the plant was powdered and 0.1 gram of it was added to the first of the five wells. One (1) mL of the mixture was added to the vials with different concentrations. Fifteen brine shrimp
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several means of protection of the body against pathogens is the activation of B cells and their generation of antibodies. These antibodies can be involved in four main functions: neutralization‚ opsonization‚ complement fixation and mediation of cytotoxicity. Neutralizing antibodies are the ones that block pathogens from gaining access to the cell by binding to receptors that the pathogen uses for this purpose. In other words‚ certain viruses like HIV and influenza express specific proteins that are
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2.3. Method Development 2.3.1. Preparation of Stock Solution 100 mg of the synthesized compound was made up to 100 ml into a 100ml standard flask with ethanol to give a solution of 1000 μg/ml. 2.3.2. Determination of λ max 1 ml of stock solution was pipetted out was made up to 10 ml into a 10ml standard flask with ethanol to obtain strength 100 μg/ml and scanned at 200-400nm using a UV spectrophotome-ter (Deepak V Bageshwar et al.‚ 2010). 2.3.3. Preparation of Standard Calibration Curve Aliquots
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many herbs are considered safer than conventional medications. The researcher will try to create herbel remedy for Urinary Track Infection using the extract of the natural fruit of Bignay‚ antidesma bunius as scientific name‚ that contains cytotoxicity to help person with urinary track infections. Statement of the Problem: This study sought to answer the following problems: 1. Can we produce a herbal medicine out of the extract of the natural fruits of bignay for
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Abstract Bone graft procedures‚ in particular maxillofacial repair‚ account for half of the orthopedic procedures done in the US each year. Infection is a major issue in surgery‚ and should be of primary concern when engineering biomaterials. Silver is of renewed importance today‚ as it has the ability to potentiate antibiotics against resistant bacterial strains. In order to reduce long term infection risks‚ it is necessary for the scaffold to maintain a silver ion release for the length of the
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SUMMARY AND CONCLUSION 6.1 Summary Silver nanoparticles are nanoparticles of silver composed of a large percentage of silver oxide due to their large ratio of surface to bulk silver atoms. Various shapes and sizes of nanoparticles are synthesized by physical‚ chemical and biological methods. The Chrysophyllum canito leaf extracts were used for silver nanoparticles synthesis. In this study utterly revealed the best milli molar concentrations of silver nanoparticles synthesis in diverse solvents such
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cells were cultured in 24-well plates inoculated with the virus (105 PFU mL-1) and treated simultaneously with varying concentrations of the samples (25‚ 50‚ 100 and 200 μg mL-1 for AcF and 50‚ 100‚ 200 and 290 μg mL-1 for FM45) determined by the cytotoxicity test. DMEM (2x) containing 25 mM MgCl2 and agarose (1.8%) was added to the cells and then incubated at 37°C with 5% CO2. After 72 h‚ fixation with 20% formaldehyde in PBS (pH 7.3) was done for 24 hours. To count the plaque forming units (PFU)‚
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way of performance‚ were sensitive and definitely time consuming. Finally‚ N-terminal modification with 5(6) Carboxyfluorescein (FAM) in sequences was used in uptake studies and Pyroglutamic acid (pGlu) in sequences was chosen in order to use in cytotoxicity and cell viability assays. On the other hand‚ C-terminal modification was done by applying Memantine (Mem) in sequences used in
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correctly‚ many herbs are considered safer than conventional medication. The researchers tried to create herbal remedy for Urinary Track Infection using the extract of the natural leaf of Bignay‚ antidesma bunius as scientific name‚ that contains cytotoxicity to help person with urinary track infections. B. Statement of the problem: This study sought to answer the following problems: 1. Can herbal medicine be produce out of the extract of the natural leaves of bignay for a person with urinary
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For the treatment of cancer most of the drugs currently available but they have limited therapeutic potential because they are very toxic‚ expensive‚ highly inappropriate action in treating cancer‚ or having so many side effects like granulocytopenia‚ agranulocytosis‚ lymphocytopenia‚ alopecia‚ carcinogenicity or secondary cancer especially leukaemias and hyperuricaemia etc. Curcumin is one such natural herbal medicine‚ derived from the extraction of dried rhizome of turmeric or Curcumin longa‚
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