WESTERN BLOTTING (Written Report) I. PRINCIPLE Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight. In Western blotting‚ proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a
Premium Western blot Molecular biology Gel electrophoresis
The Western Genre Many movies come out and pertain to a certain genre. The western genre is an example of that. But then there raises a question‚ what exactly makes this movie a western film? There may also be the question‚ what can the viewer expect to see in a western film? We’ll to start by answering the first question. Western films all have similar plot lines‚ objects‚ and elements within the genre that will be extend to most if not all other western movies. For example‚ true westerns typically
Premium Native Americans in the United States United States Cowboy
The Western Ghats or the Sahyādri constitute a mountain range along the western side of India. It is a UNESCO World Heritage Site and is one of the eight hottest hotspots of biological diversity in the world. It is sometimes called the Great Escarpment of India. The range runs north to south along the western edge of the Deccan Plateau‚ and separates the plateau from a narrow coastal plain along the Arabian Sea. The range starts near the border of Gujarat and Maharashtra‚ south of the Tapti river
Premium Western Ghats
there a heaven and hell? Eastern and Western philosophy are similar but different in many ways. Eastern philosophy includes the various philosophies of ancient China and India‚ but can also include Islamic‚ Jewish‚ and Persian philosophies. Western philosophy refers to the philosophies developed by the ancient Greeks and Indians. There are many differences between Eastern and Western philosophy. One is the east uses concepts by intuition while the west uses
Premium Philosophy
Name: Tutor/ professor: Institution: The western tradition Introduction Renaissance is a French term meaning rebirth is a perfect description of the economic as well as intellectual changes that happened in Europe continent from fourteenth century through the sixteenth century. In the course of the renaissance era‚ the whole of Europe sprung from stagnation in terms of economic status which was dominant in the middle ages to a period of economic or financial prominence. Of much greater importance
Premium Renaissance Protestant Reformation Middle Ages
1.0 Front office department 1.1 Organisational chart [pic] 1.2 Duties of front office staff 1.2.1 Front office manager It is the basic function of the front office manager to directly supervise the front desk ‚ uniformed service the front desk ‚ uniformed services‚ PBX (private branch exchange ) and reservation departments on daily basis and to monitor guest services : (Deveau L.T. et.al‚1996).The person who responsible of all the function of the front office. Basically
Premium Telephone exchange Hotel Telephone
Weather Fronts: Understanding the Movement of Air Lesson Plan on types of weather fronts with various conceptual demonstrations By: Craig O’Connell and Jack Hord A. State Standards Addressed a. Standard 6.1: The student will demonstrate an understanding of technological design and scientific inquiry‚ including process skills‚ mathematical thinking‚ controlled investigative design and analysis‚ and problem solving. i. Indicator 6-1.1: Use appropriate tools and instruments
Premium Hot air balloon
Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish. Chae Yee Kent State University: BSCI 30140 Lab Abstract Western Blotting can be used to detect the Myosin actin light chain in different species of fish and is used to distinguish from different species based on variation‚ commonality‚ or evolutionary divergence. First‚ proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according
Premium Molecular biology Protein Gel electrophoresis
antiquity that exists in the Eastern cultures. We think 300 years is a long time when in Europe traditions can be traced back a couple thousand years and in India they can trace Hinduism and their way of life back 4‚000 years. The very nature of western religion is nothing but change. It has its roots in Greek and Roman polytheism and to go from polytheistic to monotheistic is a huge‚ 180 degree turn and about the biggest change
Premium Religion Hinduism Islam
Air Masses and Fronts Air masses is every where around us‚ it creates pressure around our body and keeps us from floating. Air mass is the pressure in the gravity that also has to the temperature in the atmosphere to create different pressure that effects you in different ways. For example‚ if hot air were to fill a plastic bag it would rise‚ but if it where cool air that filled the bag it would be less likely to rise than the other. Fronts‚ on another hand‚ are produced when a large amount of
Premium Precipitation