the decolorized 2. Dependent variable The concentration of DCPIP solution 3. Controlled variable The concentration of DCPIP solution The amount of fruit juice The temperature The time that the juice expose to the air Section C. Introduction 1. Background information DCPIP is an enzyme-catalyzed oxidation electron acceptor that is pink in its oxidized form. In normal condition‚ DCPIP is blue dyed. When DCPIP is added into vitamin C solution‚ the vitamin C reduces the dye
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a solution of DCPIP. This was done by using the concentration‚ absorbance‚ wavelength values. 2. The purpose of the experiment was to determine the concentration of an unknown DCPIP sample by measuring the absorbance maximum of a specific concentration of DCPIP. 3. There was no hypothesis. We were merely trying to replicate the experiment. Procedure:
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Vitamin c in fruit juice using dcpip titration Preparing the standard ascorbic acid solution 1. Weigh out accurately about 0.2 g ascorbic acid and make up to 1 L with distilled water. 2. Calculate the concentration of the ascorbic acid solution: C = n/V = m/M/V. Sample calculation: mass of ascorbic acid = 0.205 g C (ascorbic acid) = 0.205/176.12/1.00 = 0.00116 M Preparing the DCPIP solution Weigh out accurately approximately 0.24 g DCPIP (Mr = 268.1g/mol) and make up to 1 L with
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reversible. Vitamin C is a good reducing agent and therefore it is easily oxidised. Vitamin C can be detected by titrating it against a solution of an oxidizing agent. A common type of oxidizing agent that is used to test for presence of Vitamin C is DCPIP solution. Structural formula of vitamin C SOURCES OF VITAMIN C All fruits and vegetables contain some amount of vitamin C. Foods
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juice needed to decolourise 1cm3 Of 1% DCPIP solution. DCPIP solution can be used to measure the amount of vitamin C in each juice as an antioxidant (like vitamin c) reduces the blue dye‚ DCPIP changing it from blur to colourless. Method 1) Pipette 1cm3 Of 1% DCPIP solution into a test tube 2) Fill a burette with the 1% vitamin C solution and position it above the test tube with DCPIP‚ allowing the vitamin C solution to decolourise the blue DCPIP drop by drop‚ shaking the test tube gently
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Title : The Vitamin C content of fruit juice Aim : To investigate the vitamin C content of fruit juice. Introduction : [2]Vitamin C‚ also known as the ascorbic acid or the L-ascorbate‚ is required for the growth and repair of tissues in all parts of our body. It is necessary to form collagen‚ an important protein used to make skin‚ scar tissue‚ tendons‚ ligaments‚ and blood vessels. Vitamin C is essential for the healing of wounds as well as for the repair and maintenance of cartilage‚ bones
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To investigate the vitamin C content of fruit juice.Hypothesis: I believe the more drops of fruit juice taken to decolourise DCPIP‚ the smaller the amount of vitamin C in that fruit juice. This is because DCPIP needed to be decolourised by ascorbic acid in fruit juice‚ so less concentration of ascorbic acids means that more ascorbic acids is needed to decolourised DCPIP. I believe that freshly squeezed orange is going to have the most Vitamin C content because the fruit itself had a higher average
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vitamin c levels claimed by manufacturers are actually present in the drinks‚ this will be done by experimenting various drinks using blue DCPIP solution. The quantity of vitamin C in food and drink can be determined using a simple colour test. Vitamin C decolourises the blue dye DCPIP (dichlorophenolindolphenol). Vitamin C is an antioxidant and reduces the DCPIP. DCPIP changes from blue to colourless (or slightly pink) as it becomes reduced. Hypothesis: Is High vitamin C all it claims to be?
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decolourise DCPIP solution‚ the higher vitamin C content in the fruit juices itself. The fresh orange juice gains the highest vitamin C content than other juice (carton juice‚ fresh lime and fresh lemon). NULL HYPOTHESIS All type of fruit juices has the same value of vitamin C content. VARIABLES Manipulated Variable: Type of fruits juice used Responding Variable: Volume of Fruit juices decolourise DCPIP solution Control Variable : volume of DCPIP solution‚ Concentration of DCPIP solution
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pipette‚ 3 small beakers‚ DCPIP solution‚ 0.1% ascorbic acid‚ distilled water‚ 4 varieties of fruit juice‚ for example mango‚ grape‚ apple‚ guava etc. Preparation Take a properly washed beaker and make 0.1% solution of vitamin C or ascorbic acid with 0.1 g of vitamin C in 100 cm3; this is 10 mg cm-3 or one may also use a readily prepared 0.1%ascorbic acid. Take a 0.1% solution of DCPIP. Take 6 test tubes and label them
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