using iodine test. 4) To test the presence of lipids in corn oils when using the Sudan Ш and Emulsion tests. 5) To test the presence of proteins in an egg albumen in the Biuret’s test. 6) To test the presence of Vitamin C and ascorbic acid in the DCPIP test. Results: Figure 1 :Results of biochemical test for different food samples in test tubes In test tube A‚ the Benedict’s test is used to test for the presence of reducing sugar which is glucose.This test gives the positive result and
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determine how the relative redox activity of chloroplasts from spinach leaves‚ which were performing photosynthesis‚ would change when in the presence or absence of light. To observe these changes in redox activity‚ the chloroplasts were exposed to DCPIP‚ a chemical that changes color according to such activity. By determining the redox activity of the chloroplasts‚ it could then be inferred which chloroplasts were photosynthesizing more actively than others. Redox activity‚ otherwise known as oxidation-reduction
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Biology SL Internal Assessment What is the effect of heat on vitamin C concentrated in lemon juice? Introduction: “Vitamins are complex organic substances that are needed in very small amounts for many of the essential processes carried out in the body.” 1It is estimated and supported scientifically that only a few mg are filling the daily recommendation‚ which is essential for a healthy living. As most of vitamins cannot be produced by our bodies‚ we need to obtain them
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Absorbance at 620 nm for each treatment DCPIP + chloroplasts t=0 min. 0.93 0.945 0.905 0.915 t=3 min. 0.95 0.731 0.83 0.816 change in A620 -0.02 0.214 0.075 0.099 t=0 min. t=3 min. change in A620 t=0 min. t=3 min. change in A620 0.632 0.636 0.636 0.628 0.63 0.634 0.004 0.006 0.002 0.476 0.416 0.422 0.478 0.417 0.44 -0.002 -0.001 -0.008 Filter Colour Blue Green Red 2 DCPIP+ 5ml of sorbitol Corrected change in A620
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Abstract : The purpose of the experiment was to determine the wavelength and light intensity effect on the reaction rate of photosynthesis. Photosynthesis is the conversion of light energy to chemical energy in the form of organic compounds. There are two phases in photosynthesis‚ the light reaction and dark reaction. The following experiment tested only the light reaction. Photosynthesis takes place on the chloroplast‚ and the chloroplast contains chlorophyll pigments which absorb light energy
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Cooking oil Egg white solution Distilled water E Test for proteins using Albustix paper Sample Albustix paper Original colour Final colour Egg white solution Distilled water F Test for vitamin C using DCPIP solution Sample DCPIP solution Original colour Final colour Vitamin C solution Boiled vitamin C solution Distilled water Questions: 1. Do the amount of samples used in food tests need to be measured very accurately?
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fruits‚ orange‚ lemon and lime‚ and to compare them and find out which fruit contains the highest concentration of Vitamin C. By using the juice from each fruit to make a solution to combine with 10cm³ of DCPIP until the DCPIP turned colourless and recording the amount of Vitamin C needed to make the DCPIP colourless‚ the molarity of Vitamin C in each fruit could be calculated and it was found that lemon has the most amount of Vitamin C followed by lime and orange. Research Question/Hypothesis/Introduction
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Principle: Test for lipids using the grease spot test Test for glucose using Clinistix paper Test for reducing sugars using Benedict’s test Test for starch using the iodine test Test for proteins using Albustix paper Test for vitamin C using DCPIP solution A control using distilled water instead of the sample should be included. Independent: different types of food samples Dependent: different types of food substance presence in food sample Assumption: Controlled: using the same amount
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generating ATP (Hoober 1984). The chemical DCPIP acts as an electron acceptor and is used to measure the rate of electron transport in the thylakoid membrane of chloroplasts (Dean and Miskiewicz 2006). Initially DCPIP is a blue colour. Although‚ when it gains electrons from the transport chain it is reduced and turns colourless (Dean and Miskiewicz 2006). A high photosynthetic rate can be interpreted by a fast rate of change from blue to colourless of the DCPIP as more electrons are flowing through the
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and ATP is done through the light reactions. Light independent reactions use carbon dioxide and the products of light reactions (ATP and NADPH) to form compounds such as glucose. The rate of photosynthesis can be determined indirect by the indicator DCPIP. When reduced this indicator changes from blue to a colorless solution. When light is absorbed‚ water is oxidized and the excited electrons are transferred for the process
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