of hydrogen peroxide (H2O2) in the body by breaking it down into water and oxygen gas. Hydrogen peroxide is a toxic by-product of metabolic reactions. If hydrogen peroxide accumulates in the cells‚ it disturbs the function of cells so it is important to get rid of this toxic chemical from the body. One way to avoid the accumulation of H2O2 is to convert it water and oxygen which can be used in other reactions in the cell. In the
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substrate concentration. (Clegg‚ 2007) Materials: Fresh liver (will be cut into 12 small cubes with equal size). Frozen works fine | Thin spatulas to scrape liver from mortar into test tube | 4 test tubes and stand | Fine sand | 3% hydrogen peroxide | 100mL breaker | Mortar and pestle | 10 mL Measuring cylinder | Bunsen burner/hot plate | Detergent | 1M Hydrochloric acid (HCL) | Ruler | Matches | Knife | Procedure: 1. Cut 12 small cubes of liver (under 1 cm)‚ of equal size
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of hydrogen peroxide Potatoes Raw potato Potato soaked in baking soda Potato soaked in baking soda Stopwatch Goggles Procedures: 1. Get all your materials. Put all the potatoes into individual test tubes. 2. Pour 3mL of hydrogen peroxide into one test tube. Watch and time how much time goes by before the reaction begins. 3. Observe and write down the time. 4. Repeat steps 2 and 3 until all the potatoes have been tested and observed. Data: Type of potato Amount of hydrogen peroxide
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McGonegal‚ Patricia Tosto‚ and William Ward. Pearson Baccalaureate: Standard Level Biology for the IB Diploma (Pearson International Baccalaureate Diploma: International Editions). n/a: Imprint Unknown‚ 2008. Print. “Effect of Catalase on Hydrogen Peroxide.” http://www.sciencegeek.net/Biology/biopdfs/Lab_Catalase.pdf. (25 Jan. 2013). (Add your own references)
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between temperature‚ and the rate of enzyme-catalase reaction. Catalase is an enzyme that reduces the amount of activation energy required to break down hydrogen peroxide (H2O2)‚ a by-product of oxygen metabolism‚ into water (H2O) and oxygen (O) (http://www.wisegeek.org/). This reaction is refered to as the enzyme catalyzed reaction. Hydrogen peroxide substrate binds to the active site of the enzyme stressing the bonds‚ ultimately resulting in a release of oxygen and water. An increase in temperature
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the pH (concentration of hydrogen ions)‚ remain within a fairly narrow range. Extreme variations in pH and temperature denature the enzyme by altering its chemical structure‚ thus adversely affecting the chemical reaction. As even the slightest change in the protein’s structure will change the enzyme’s shape enough to prevent the formation of the enzyme-substrate complex‚ the reaction will then be unable to take place. The enzyme‚ catalase‚ reacts with hydrogen peroxide in order to yield water and
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Chemistry: It is the science concerned with the atomic composition of substances‚ elements and their interactions‚ and the formation‚ decomposition and properties of molecules. -Biological chemistry or Biochemistry is the biological or physiological chemistry of living organisms and of the chemical changes occurring therein. Structure of Matter: All living and nonliving things are composed of matter. Matter; is anything that occupies space and has mass. Mass is the amount of matter in an object
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of this lab are used in this part‚ but in contrast to part one this part will vary the pH of the enzyme solution. The enzyme‚ again‚ is catalase‚ the substrate is hydrogen peroxide‚ and the products are the oxygen bubbles that are produced. Also‚ in this lab the time it takes for each disc to rise to the surface of the hydrogen peroxide solution determines which pH the reaction occurs the fastest at. So‚ the lowest average number of seconds for the disc to return to the surface of the solution at
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person taking the measurements may not have measured the height at exactly 20 seconds but a few moments after. · Reading and recording errors: There are errors surrounding the reading of measurements of the hydrogen peroxide solution and of the height of the froth. If the hydrogen peroxide solution was measured incorrectly by not being taken at eye level‚ this could have affected the rate of the reaction by having a varied amount of solution added to the liver. If the froth was measured incorrectly
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the research question; do changes in temperature (from 0˚C‚ 7˚C‚ 21˚C‚ 37˚C‚ and 90˚C) have an effect on the activity of the enzyme catalase (found in beef liver) in the breakdown of hydrogen peroxide? Hypothesis If liver is placed in different temperatures of 0˚C‚ 7˚C‚ 21˚C‚ 37˚C‚ and 90˚C and hydrogen peroxide is added to each piece of liver‚ then the liver placed in 37˚C will have the largest reaction height. The liver in 90˚C will have the smallest reaction height‚ followed by the liver
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