by speeding up the time it takes for the reaction to occur‚ without getting “used up” throughout the process. Catalase‚ the specific enzyme used in this lab‚ is a protein abundant in the liver and red blood cells. It enhances the breakdown of hydrogen peroxide‚ the substrate‚ into oxygen‚ the element measured in the lab‚ and water (Wiles‚ 2016). This lab included three different procedures‚ all preformed with an assay system‚ in order to gather information on the kind of environment ideal for catalase
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activity. Catalase by scavenging hydrogen peroxide to water and oxygen is an important enzyme of cell defense mechanisms against oxidative stress in all living organisms (Dat et al.‚ 2003). Cells use catalase because it is the most efficient enzyme as an antioxidative enzyme which lowers hydrogen peroxide or superoxide to accumulate to toxic levels in plant growth (Bowler et al. 1992). The formula that involves catalase as the enzyme is . pH is a measure of the Hydrogen ion concentration of a solution
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There are so many things to learn as a kid. You learn to go pee on the potty‚ ride your bike and brush your teeth. You are taught to brush your teeth at a very young age. It seems like a rather simple task‚ yet there are so many variables. There are many types of toothbrushes out there. There is the standard toothbrushes that are approved by the American dental association. Some of the most popular brands approved are Philips‚ Crest‚ Sensonic‚ Braun‚ Sonicare‚ Colgate‚ Oral-B‚ and Reach. There
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over and over (Perry‚ Morton 2007). Catalase is a specific enzyme that is found in many animal and plant tissues (Sullivan‚ 2013). It is used to speed up reactions that break down hydrogen peroxide. For example‚ the enzyme is catalase and the substrate is hydrogen peroxide (Lab Report). Hydrogen peroxide is a byproduct of numerous cellular reactions that happen in our body‚ however this byproduct is very toxic to our
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(Cheeptham & Lal‚ 2007). If it is catalase positive it will produce bubbles with the drops of hydrogen peroxide on the bacteria. This will show if the bacteria is an aerobic or aero tolerant anaerobes or true anaerobes. Catalase is an enzyme that will take hydrogen peroxide and break it down to water and oxygen.(Merriam Webster 2013) “Catalase is thought to be a major defense against hydrogen peroxide” (Eaton & Ma‚ 1992). We will also determine if the bacteria is stable or non-stable acid fermenters
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Experiments investigating the effect of a particular factor on an enzyme-catalysed reaction are commonly used as assessed practicals in Biology. The reason for this is there are a number of factors that can be investigated‚ and therefore‚ a number of factors that can also be controlled. From our lecture‚ we are aware that the following can affect the rate of reaction: 1) Temperature: a. Independent variable: A range of temperatures should be investigated‚ with particular attention
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Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10‚ 37‚ 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start
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accumulation of toxic levels of hydrogen peroxide formed as byproducts of metabolic processes.
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INTRODUCTION The enzyme catalase converts hydrogen peroxide (H2O2) to water and O2. The evolution of O2 causes bubbling. Thus‚ catalase-positive organisms that are mixed into hydrogen peroxide will cause bubbling (catalase-negative organisms will not). This test is good for distinguishing between Gram-positive cocci in chains (catalase negative) versus Gram-positive cocci in clusters (catalase positive). The coagulase test is used to differentiate Staphylococcus aureus from coagulase-negative
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Table of Contents: CHAPTER I: Introduction . . . . . . . . . . . . . . . . . . . . . . .i Statement of the problem . . . . . . . . . . . ii Hypothesis . . . . . . . . . . . . . . . . . . . . . . . . iii Scope and limitation . . . . . . . . . . . . . . . iv Definition of terms . . . . . . . . . . . . . . . . . v CHAPTER II:RRL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi CHAPTER III:Methodology . . . . . . . . . . . . . . . . . . . . . . vii CHAPTER IV:Results
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