negative organism‚ the zone of inhibition was the largest with Lysol (0.2mm). Next‚ Scope has slightly smaller zone of inhibition‚ 0.15mm‚ on the organism. Hydrogen peroxide‚ Wescodyne and 70 percent Ethyl Alcohol had no affect on the bacteria. Inhibition of S. aureus was exhibited by Hydrogen Peroxide (0.19mm) and Lysol (0.13mm)‚ Hydrogen Peroxide having the largest inhibition zone. Scope‚ Wescodyne‚ and 70% Ethyl Alcohol were negative for having inhibiting capacity. From these results‚ it can be
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In this experiment the substrate is going to be hydrogen peroxide(H2O2) and the enzyme is going to be catalase. Hydrogen peroxide(H2O2) is a product of aerobic cellular respiration and is considered a free radical. This means that too much of hydrogen peroxide can affect the organelles. In order to stop damages caused by hydrogen peroxide‚ the enzyme catalase changes hydrogen peroxide(H2O2) to water(H2O) and oxygen gas(O2). Catalase is present most animal cells. The
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pipette • Ice bath • 0.1% hydrogen peroxide solution • 3 thermometers • Liver puree • Warm-water bath • 25-mL graduated cylinder • Clock or watch with second hand • 5 50-mL beakers • Paper towels • Filter-paper disks Part A: Observe the Catalase Reaction 1. Put on apron and safety goggles. Use forceps to place a small piece of raw liver in an open petri dish. Use a dropper pipette to put a drop of hydrogen peroxide solution on the liver. CAUTION: Hydrogen peroxide can be irritating to skin
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water. B. Cereus survived 10 seconds‚ 30 seconds‚ 60 seconds‚ and 300 seconds of emergence in boiling water. Effectiveness of Antibacterial Products on S. Marcescens Product Tested Effectiveness Lysol with Bleach +++ Bactine + Hydrogen Peroxide +++++ Neosporin + Purell Germ-X
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organelles and tissue damage by peroxide. Catalase is normally found in the liver and in our case‚ it is found in the lamb liver (EEB‚ 2015). The equation for this reaction is 2H2O2 → 2H2O + O2‚ where hydrogen peroxide is broken down into oxygen and water molecules (McDowall‚ 2004). Temperature has an effect of the structure of the enzyme and the hydrogen bonds. The optimal temperature for catalase is 37˚C. As temperature increases towards the optimal temperature‚ the hydrogen bonds loosen and this makes
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reacts with the hydroxide molecules to form two negative charges‚ or dianion. The dianion then reacts with oxygen to form a 5-aminophtalic acid and nitrogen gas. After‚ the oxygen forms with the hydrogen peroxide and the
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Another redox colour change takes place when potassium iodide‚ which is colourless‚ is oxidised by an oxidising agent to form brown iodine solution. An example of this reaction is potassium iodide solution reacting with a solution of hydrogen
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Purpose: The purpose of this lab is to find out which substance‚ alcohol‚ antibacterial soap‚ water‚ or hydrogen peroxide‚ is the best at preventing the growth of bacteria. Hypothesis: Antibacterial soap will be the best because soap is a substance that people use to wash their hands because it takes away the germs. Even the name‚ antibacterial soap‚ suggests that it fights off bacteria. Hydrogen peroxide is second because it is used to kill off cells on wounds and is poisonous. Rubbing alcohol will be
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increasing the kinetic motion of molecules‚ changing the rate of collisions between them and the hydrogen bonds it’s 3D structure(D. Fraser‚ 55). There is a specific temperature for every enzyme where activity is maximized‚ and also where an enzyme becomes denatured. An enzyme becomes denatured when it is heated at extreme temperature; the excessive kinetic movement of the amino acid begins to break the hydrogen bonds holding its 3D structure together‚ typically around 70℃. Another factor that can affect
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mortar & pestle‚ 4 test tubes‚ test tube rack‚ spatula‚ hydrogen peroxide 6%‚ 3%‚ 1.5%‚ pipettes‚ scalpel‚ ruler. Procedure 1. Cut using scalpel several raw potato cubes each measuring 1 cubic centimeter (cc). 2. Each cube was ground with a mortar & pestle and then added to a test tube using a spatula. 3. Test tube 1 was left as the control with no added hydrogen peroxide. 4. Different concentrations of hydrogen peroxide solution were added to each test tube using a pipette. (5mL
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