Electrophoresis of Amino Acids Introduction: Electrophoresis is a separation technique based on the movement of charged ions under the influence of an electrical field. This technique is primarily used for the separation of amino acids and peptides on the basis of their charge. All amino acids contain ionizable groups that cause the amino acids‚ in solution‚ to act as charged polyelectrolytes that can migrate in an electric field. The amino acids with a net positive charge will migrate toward
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Abstract: Introduction: Materials: * Chemicals: Buffer solution‚ pH 7.0‚ 50 mL Phenolphthalein indicator solution‚ 1.0 %‚ 1 mL Potassium hydrogen phthalate‚ KHC8H4O4‚ 2 g sodium hydroxide solution‚ NaOH‚ 0.1 M‚ 150 mL Unknown weak acid‚ 1.5g Water‚ distilled or deionized * Equipment: Balance Stir bar Beaker‚ 250mL Oven Buret‚ 50 mL pH sensor Desiccator Rising stand and buret clamp Erlenmeyer flask‚ 125mL Wash bottle with distilled water Funnel Weighing dishes‚ 2
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Experiment Acids‚ Bases‚ and Neutrals Problem Are common house-hold products pH balanced and neutral? Information Gathered pH measures how acidic or alkaline a solution is. The pH scale ranges from 0-14. Solutions that range from 0-6 are considered to be acidic. Solutions that are on the acidic end of the scale are low in pH‚ high in hydrogen ion‚ and low in hydroxyl ions. An acid has a sour taste‚ hence‚ will react to metal and will be corrosive; therefore‚ they are considered as an electrolyte
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9.1 Sulphuric Acid Sulphuric Acid is Manufactured in Industry 1. Sulphuric acid‚ H2SO4 is manufactured in industry through Contact Process. 2. The raw materials used are sulphur‚ air and water 3. The Contact process consists of three stages. Stage 1: The production of sulphur dioxide This can be obtained by two methods: 1. Burning of sulphur in dry air in the furnace S + O SO2 2. Burning of metal sulphide such as zinc sulphide or iron(III) sulphide in dry air. 2ZnS
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ACID/BASE You might need to know the following K values: CH3COOH Ka = 1.8 x 10–5 Benzoic Acid Ka = 6.5 x 10–5 HNO2 Ka = 4.5 x 10–4 NH3 Kb = 1.8 x 10–5 HF Ka = 7.2 x 10–4 H2S Ka = 5.7 x 10–8 HSO4– Ka = 1.2 x 10–2 HS– Ka = 1.2 x 10–13 HCOOH Ka = 1.8 x 10–4 HOCl Ka = 3.0 x 10–8 SIMPLE ACIDS AND BASES 1. According to the Brønsted–Lowry definition‚ which species can function both as an acid and as a base? (A) Cl– (B) SO42– (C) NH4+ (D) HCO3– (E) H3O+ 2. Which of the following
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to practice common organic laboratory techniques inside the lab to get one oriented to the basic methods of procedure that can be used for later experiments. This experiment involves the separation of benzoic acid from a more crude form‚ consisting of benzoic acid‚ methyl orange‚ a common acid/base indicator‚ and cellulose‚ a natural polymer of glucose (Huston‚ and Liu 17-24). The technique that is used to perform this separation is called extraction. Extraction is a systematic process of separating
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STANDARDIATION OF ACID AND BASE Eunice Ivy B. Gamboa ABSTRACT Solutions of known concentration are prepared by dissolving measured masses of standard acids in distilled water. The concentrations of unknown solutions of sodium hydroxide are determined by titration. An acid solution reacts with a base solution in a "neutralization" reaction. Titrations permit the concentrations of unknown acids/bases to be determined with a high degree of accuracy. In order to analyze unknown acids/bases‚ we must have
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Oxalic Acid Lab Aim: Use acid base titration to determine the number of water molecules in hydrated hydrochloric acid. Apparatus required: Oxalic acid solution 250 cm3 Weighing bottle Digital balance Beaker (250 cm3) Distilled Water Volumetric Flask 250cm3 Filter funnel Pipette Burette 50cm3 Retort Stand Beakers 100cm3 Standardized sodium hydroxide solution 0.1M Pipette filter Conical flasks 250cm3 Phenolphthalein Indicator Procedure 1) Rinse the burette with distilled
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EXPERIMENT 2 ACID DISSOCIATION CONSTANT OF AN INDICATOR DYE OBJECTIVES Using spectrophotometric method: determine the wavelengths at which the acid and base forms of the dye in aqueous medium exhibit maximum absorption; determine the molar absorptivities of the acid and base forms of the dye and estimate an unknown concentration of the dye in solution using the Beer-Lambert’s Law; and determine the acid dissociation constant of the indicator dye. THEORY The absorption or reflection of
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expected melting point. After its temperature reached the plateau‚ the temperature increment was set at 1°C per minute‚ and the melting point range of 152°C-154°C was recorded. After standardizing the melting device‚ a small sample of unknown carboxylic acid was placed in a capillary tube‚ and an arbitrary plateau of 200°C was set. When the sample started to melt at 150°C‚ another capillary tube containing a small amount of unknown sample was placed in the melting device‚ and the plateau was set to 135°C
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