To examine three different ways of denaturing proteins. HypothesisIt was predicted that the physical characteristics of the egg white solution at room temperature would appear clear and normal like a raw egg white. This is because nothing would be done to the egg white. It was also predicted that when the egg white solution gets heated‚ the protein would denature if the temperature exceeds 65 °C. The protein would solidify‚ turn opaque‚ and turn white in colour. This would happen because the heat
Premium Protein Egg white
Analytical Chemistry Experiment 2b: Determination of the ASA Content of Aspirin Due Date: September 16‚ 2013 Experiment#: 2b Title: Determination of the ASA Content of Aspirin Aim: To determine the Molar Concentration of NaOH and HCl acid used in their Standardization processes and to determine the acetylsalicylic acid (ASA) content in Aspirin. Materials/Apparatus: materials used are the same as that outlined in the laboratory procedure prepared by the laboratory instructor. Procedure:
Premium Aspirin Acetic acid Salicylic acid
Biological Membrane Study: HCl‚ NaOH‚ SDS Objective and Hypothesis: The objective of this lab is to determine the effects of SDS‚ HCl‚ and NaOH on red cabbage cell membrane. Prior knowledge can tell us that the red cabbage is a red-purple color due to a pigment called anthocyanin. This is what we will be testing in the experiment. We also know that HCl is a strong acid (very low pH) and that NaOH is a strong base (very high pH). From information learned in this course (lecture/lab) we can assume
Premium Chemistry Cell membrane Cell
Denaturation (biochemistry) Denaturation is the alteration of a protein shape through some form of external stress (for example‚ by applying heat‚ acid or alkali)‚ in such a way that it will no longer be able to carry out its cellular function. See also: Plants & Animals Cell Biology Genetics Molecular Biology Matter & Energy Biochemistry Organic Chemistry Thermodynamics Denatured proteins can exhibit a wide range of characteristics‚ from loss of solubility to communal aggregation. Proteins
Premium Protein Amino acid
Chemistry SL! Research Question! How is the solubility of sodium hydroxide (NaOH) in water affected by temperature? ! ! Introduction ! Sodium hydroxide is categorised as metal halide salt‚ composed of sodium and chlorine.! The ions present in the solid crystals of potassium chloride dissolve and gain mobility in water. When potassium chloride is dissolved in water‚ the following endothermic reaction occurs:! NaOH(s) + H2O(l) → Na+(aq) + OH–(aq) + H2O(l)! This reaction takes place because
Premium Sodium chloride Solvent Water
Introduction Protein purification is the series of processes to isolate a single type of protein from a complex mixture. This is vital to extract and characterize the protein of interest. However‚ before doing so‚ it is important to release the protein from the subcellular organelles. This step is also known as homogenization. This step can be done with the use of blender. As the solution was homogenized‚ it may undergo saltation or acidation to remove impurities such as calcium anions. Hexane
Premium Protein Coomassie Enzyme
Procedure: A 0.170g sample consisting of a mixture of 0.110g of an unknown neutral compound and 0.060g of benzoic acid was mixed with ether and shaken until all solids dissolved. NaOH was added‚ shaken‚ allowed to separate and the bottom layer removed and transferred to a test tube labeled “NaOH 1st extract”.
Premium Chemistry Sodium hydroxide Acetic acid
HCL TECHNOLOGIES In 2011‚ HCLT ERS (Engineering and R&D Services)‚ a division of HCL Technologies‚ a global IT services corporation headquartered in New Delhi‚ India‚ had to devise next year ’s plan for the Engineering Out Of The Box (EOOTB) business concept that it had initiated in 2009‚ which transformed the division ’s ability to create "16 productized solutions" and to engage new and old customers in new revenue services. The productized solutions were heavily reliant upon IT platform-based
Premium Cloud computing Product management Innovation
A STUDY ON CORPORATE SOCIAL RESPONSIBILITY (CSR) IN HCL TECHNOLOGIES‚CHENNAI Review report - 1 ( Submitted by Muralidharan.P‚ Roll No: 1110MBA0920‚ Reg No:68512150002 ) 1 NEED FOR THE STUDY CSR has become an integral part of corporate strategy. Companies have CSR teams that devise specific policies‚ strategies and goals for their CSR programs and set aside budgets to support them. Corporate social responsibility plays a crucial role in making your brand popular not only
Premium Social responsibility Corporate social responsibility
DATE: 11/10/12 COURSE CODE: BIOL 2365 Comparative Biochemistry TITLE: Proteins and Amino Acids RESULTS: Table 1: The results of experiment 1; the Lowry Test Volume of Standard Protein/ Unknown (mL) Absorbance at 750 nm 0 0.000 0.1 0.017 0.3 0.135 0.3 0.155 0.5 0.230 0.7 0.323 0.7 0.310 1.0 0.457 1.0 Unknown 1a 0.463 1.0 Unknown 1b 0.433 1.0 Unknown 2a 0.237 1.0 Unknown 2b 0.159 Table 2: The results of Experiment 2; Ninhydrin Test Amino acid Color X
Premium Amino acid Protein