Ricin is a toxic protein found in castor oil plant seeds. The toxicity of Ricin has been known for centuries and was even used by the KGB government to assassinate Georgi Markov‚ a famous journalist who openly criticized the Soviet Union’s communist regime. The toxicity of ricin is caused by its ability to deactivate ribosomes and halt protein synthesis. Therefore‚ Ricin is categorized as a ribosome inactivating protein (RIP). There are two types of RIPs: type I and type II. Type I RIPs are usually
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experiment‚ we will be testing three different proteins to see if they denature at the same temperature. A protein is a molecule containing amino acids created in ribosomes that have many functions‚ including being an enzyme and transporter‚ structuring‚ translating genetic information‚ and serving as a channel for charged molecules to pass through the cell membrane. As a molecule‚ a protein is shaped like a coil (Food Science Basics 2014). Denaturation is when a protein’s shape changes due to the
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Module 2 Section 2 EXPERIMENT: DNA & Protein Synthesis Exercise 1 – Modeling DNA 1. List the four bases which are found in DNA. (1 pt) The four bases found in DNA are cytosine‚ adenine‚ guanine and thymine. 2. Fit any six nucleotides together to form a row‚ then list the six nucleotides in the order you used them. Work with your model pieces and try fitting the bases together to make a double strand as shown in Figure 9 of the lab manual. Which nucleotides form
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Investigation Srategy I am ivestigating the reaction of sodium thiosulphate and hydroclohric acid. sodium thiosulphate(aq) + Hydroclohric Acid (aq) --> Sodium cloride (aq) + Sulphur Dioxide (g) + Water (l) + Suplhur (s) Na(2)S(2)O(3) + 2 HCl --> 2NaCl + SO(2) + H(2)O + S The finished reaction of the 2 clear liquids is cloudy.I will use this change from clear to cloudy as an indicator as to when the reaction is finished. By using this change ‚ i can see the effect of changing other factors
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The Spectrophotometer Determination Of Protein Concentrations And The Effects Sodium Dodecyl Sulphate And Triton X-100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs‚ et.al‚ 1959). Spectroscopy is the
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unable to cleave the signal sequence amino acid. B. Band B is the cleavage of the signal sequence by the signal peptidase. The cleavage of the polypeptide weighs less then when the protein was attached to the signal sequence and before it grows in the ER lumen. This is evident from the diagram‚ since lighter proteins move father down the gel than do heavier ones. Given the length
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Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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We successfully adapted a commercially available protein detection assay for the quantiWcation of PEI. All experiments described below were performed with linear 25-kDa PEI (Polysciences‚ Warrington‚ PA‚ USA) that we routinely use for the transfection of mammalian cells [4]‚ but it was possible to quantitate other PEIs (diVerent molecular weights; branched) with this method (data not shown). The mixing of a PEI solution with the Advanced Protein Assay (APA) reagent (Cytoskeleton‚ Denver‚ CO‚ USA)
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between concentration of hydrochloric acid (HCl) and the rate of diffusion?” My hypothesis for this research question is that change in rate of diffusion will be observed when the agar blocks are placed in solutions of different concentrations of HCl. As the concentration of HCl increases‚ the rate of diffusion will also increase due to the steeper concentration gradient created. To investigate the change in rate of diffusion when different concentrations of HCl are used‚ I used agar blocks that are stained
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Experiment 11.1 Conversion of t-Amyl Alcohol to t-Amyl Chloride Using HCL October 27‚ 2011 Purpose: Alkyl halides can be prepared from alcohols by reacting them with a hydrogen halide‚ HX (X = Cl‚ Br‚ or I). The mechanisms of acid-catalyzed substitution of alcohols are termed SN1 and SN2. The “S” stands for substitution‚ the “N” stands for nucleophilic‚ and the “1” or “2” stand for unimolecular or bimolecular. Secondary alcohols react with hydrogen halides by both SN1 and SN2
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