Protein Article Research John Doe SCI 241 May 17‚ 2012 Jane Doe Protein Article Research This class has been very informative during the past few weeks. We have learned how to count calories‚ read nutrition labels‚ use a tracker to monitor our daily intake‚ carbohydrates‚ and learned about proteins and their effects. According to the readings in the text and the article proteins are the building blocks in the body. I learned that protein is found in all tissues‚ cells and organs of the body
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assays to detect the presence and absence of certain antigens or antibodies and also to quantify them when necessary. Quantification can be done in a range of microgram (µg) to nanogram (ng). The ELISA procedure takes advantage of the fact that most proteins will bind firmly to the surface of different kinds of plastic (polystyrene or polyvinyl chloride)‚ usually by hydrophobic interaction. The steps of the ELISA procedure are simple to perform. There are mainly FOUR phases in ELISA: 1. Coating
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4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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“Use of Biuret Reagent to Test for Proteins In Food” Cindy Zhou Introduction Protein is a series of amino acids that are connected together by peptide bond. The bond is located between the end of previous amino acid‚ which is the carboxyl group‚ and the head of next amino acid‚ which is a amino group. The Biuret reagent is a solution of copper sulfate and potassium hydroxide. When peptide bond are presented‚ the copper (II) will interact with them (MadSci Network: General Biology). It is
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Fish phylogenetic tree based on protein size Amanda Reed Lab partners: Lecia Redwine‚ Kyle Hatcher TA: Baneshwar Singh Biology 117‚ Section 25 Tuesday 10:30 AM Introduction Although tree diagrams have been used since the days of Charles Darwin‚ biologists have only recently adopted the tree model of evolution to read and interpret phylogenies. One of the reasons for this is the confusion that often arises from using a tree model to describe a phylogeny (Baum‚ 2008a). Many people interpret
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Bacterial Transformation Bacteria and plasmid to produce Red Fluorescent Proteins Alejandra Lopez
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April 22‚ 2015 Does protein help to build muscle? The latest craze is about protein and how it affects building muscles and building muscle strength. Today‚ there are many athletes that use it as a supplement. There are many protein supplements available that are claiming to help people who work out to build muscle faster. Protein is a structure within our bodies that consists of amino acids. The cells in our bodies need this to function properly. If we did not have protein‚ our body’s functions
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Separation of Proteins and Mass Analysis Using SDS PAGE Biology 00-01L Abstract This experiment consisted of separating proteins into polypeptides using a method called SDS PAGE which is a type of electrophoresis. The polypeptides had different masses‚ so each polypeptide traveled a different distance and this was an essential part of the lab which demonstrated that there exists a relationship between the distance traveled by the protein and the mass of the protein. This relationship was graphed
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Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
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Bio 6 (Mon. Lab) 5/6/13 The Effects of Pepsin VS. Trypsin in The Digestion of Protein Introduction As food is mechanically and chemically digested through our oral cavity then passes through our pharynx and down our esophagus‚ our food then enters the stomach. The stomach‚ being the main organ for storage‚ also helps with breaking down our food‚ but in order to accomplish that our pancreas helps by excreting an inactive digestive enzyme called pepsinogen. Pepsinogen is the inactive form
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