with acidic properties. A base reacts with acids to form salts. Titrations are used to determine the concentration of unknown substances. The purpose of the KHP experiment was to determine the molarity of NaOH. HCl titrations are mainly to check technique and used to verify the molarity of NaOH solution. The hypothesis is that this acid is C4H3OCOOH. Experimental Procedure for melting point: The identification of the melting point of the organic acid was done to find another characteristic of the
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Safety goggles. 2. Droppers. 3. Red Litmus paper. 4. Blue Litmus paper. 5. pH paper. 6. Well plate. 7. Micro spatula. Materials: 1. Zinc. 2. Magnesium. 3. Iron. 4. Copper. 5. HCL. 6. HC₂H₃O₂. 7. NaOH. 8. Phenolphthalein. Procedure: Part A: 1. Add five drops of HCL‚ HC₂H₃O₂‚ and NaOH to different depressions in the well plate. 2. Place a drop of each solution onto a piece of red litmus paper and record observations. 3. Place a drop of each solution onto a piece of
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2(22.99)+1(12.01)+3(16)g/mole = 0.2329g 105.99g/mole =2.197×10-3 moles From the equation ‚ 1 Mole Na2C03 react 2 mole HCl So‚ 2.197×10-3 moles Na2C03 = 2.197×10-3x 2 1 = 4.394x10-3 mole HCl Thus‚molarity HCl = n/v = 4.394x10-3 mole 42.87x10-3L = 0.1025 M. The suitable indicator is phenolphthalein. Primary standard
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by seeing how much drops of hydrochloric acid (HCl) are required to neutralize a certain amount of the antacid. Antacids are used to resist heartburn. We sometimes use them to treat this because antacids are a mild base that can neutralize acids in our stomachs‚ such as HCl. The purpose of this lab is to see how well each antacid neutralize hydrochloric acid. Procedure: 1. Obtain two burets‚ one for use with the HCl and others for use with the NaOH. 2. Record the exact molarity as they appear
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is sure that a reaction is occurring. The excess HCl is then diluted because of the egg shell with a drop of phenolphthalein added and is then titrated in aliquots with sodium hydroxide solution. This is the reaction for determining the excess of HCl: 2HCl (aq) + CaCO3(s) -→ Ca2+ (aq) + CO2(g) + H2O(l) + 2Cl- (aq) Materials: eggshell burette‚ 50 cm³‚ and stand pipette + filler‚ 20 cm³ plastic foil phenolphthalein indicator 0.1 M NaOH solution‚ M hydrochloric acid‚ Distilled water
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α-lactalbumin. Abstract: The Bradford assay‚ a colorimetric protein assay‚ is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250 in which under acidic conditions the red form of the dye is converted into its bluer form to bind to the protein being assayed. The (bound) form of the dye has an absorption spectrum maximum historically held to be at 595 nm. Objective: To determine the technique to a series of protein purification steps that yield α-lactalbumin in sufficient amounts
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flask add 35 ml of an Unknown HCl solution. Step 2: Add an indicator to the acid‚ select the flask and add 2 drops of phenolphthalein indicator. The indicator menu is available under the chemicals main menu (ChemicalsIndicators) or the context menu. Step 3: Fill buret with NaOH‚ obtain a 50 ml buret and fill with .100M NaOH solution. Step 4: Titrate NaOH into HCl until end point‚ record initial buret volume and add NaOH (quickly at first then slowly) until the HCl solution turns pink and record
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Abstract Titration is a laboratory method in which the endpoint of a reaction is determined quantitatively. In this experiment‚ the amount of soda ash present in a sample was determined and the alkalinity reported as it was titrated using HCl as the titrant. Also‚ the percentage amount of Na2CO3 and NaHCO3 present in the sample was determined at the end of the experiment. Introduction Soda Ash (Na2CO3) or sodium carbonate is commonly used as a cleaning agent and known as “washing
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of % Composition of Pennies Using Redox and Double Displacement (Precipitation) Reactions Introduction: Oxidation involves the gain of electrons of hydrogen or the loss of oxygen or decrease in oxidation state. If zinc completely reacts with HCL‚ then the theoretical yield of copper should be equivalent to the actual yield. Purpose: In this lab‚ we will determine the percent composition of a modern (post-1982) penny by using a strong acid to react and dissolve the zinc core‚ leaving only
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BIOLOGY LAB REPORT (UNIT 7: ENZYMES) GENERAL Enzymes are protein that acts as catalyst‚ lowering the activation energy need for reactions to progress in cells. The reaction can still occur without the presence of the enzyme‚ but at a much slower rate. The activation energy is the minimum amount of energy need for a chemical reaction to occur‚ yielding from a given set of reactants. In enzymatic reactions‚ we have substrates which are reactants of reaction bound to an enzyme. While an active site
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