Carbohydrates‚ Proteins‚ lipids‚ and nucleic Acid Lab Exercise 6 Date: 9/17/12 Bio 102-11 Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates‚ proteins‚ lipids‚ and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound. Background Most organic compounds in living organisms are carbohydrates‚ proteins‚ lipids‚ and nucleic acids they are called macromolecules
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creating better futures http://www.uow.edu.au/commerce School of Management and Marketing MGMT215: Small Business Management Subject Outline 6 credit points Subject Information Autumn‚ 2013 Southern Sydney On Campus Lecture Information: Thursdays‚ 10:30 - 12:30‚ 20.3 Pre-requisites: MGMT110 Co-requisites: Nil Restrictions: Nil Contact Hours: 2 hours edustreamed lecture plus 2 hours tutorial Online Subject Material: Nil Teaching Staff Teaching Role Name Telephone Email Room Consultation
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Determination Of Protein Concentrations And The Effects Sodium Dodecyl Sulphate And Triton X-100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs‚ et.al‚ 1959). Spectroscopy is the use of electromagnetic
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2 Production of Protein from Cloned Genes Now that we have covered the basic techniques involved in gene cloning and DNA analysis and examined how these techniques are used in research‚ we can move on to consider how recombinant DNA technology is being applied in biotechnology. This is not a new subject‚ although biotechnology has received far more attention during recent years than it ever has in the past. Biotechnology can be defined as the use of biological processes in industry and technology
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Cellular respiration is the process of breaking down chemical energy‚ most commonly glucose and oxygen‚ for use in the body as ATP with the release of water and carbon dioxide as bi-products (Mauseth‚ 2014). This process can be observed by careful analysis of the metabolic rate which is the total amount of energy released by an organism per unit mass. The respiratory quotient (R.Q) is the ratio of CO2 molecules lost per O2 molecules consumed which can be used to determine the efficiency of the metabolic
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Objective: The main objective for this lab was to use the Bradford and DC Protein Assay in order to determine our concentration of E. Coli BL21(DE3) lysate. Principle of Methods: To accomplish this objective‚ each pair of lab partners received an E. Coli lysate sample which we had to thaw by warming it with our hands and centrifuge it. We extracted a total of 12 samples with known concentrations of bovine serum albumin and 10 samples of different amounts of lysate put into them. Using the different
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lemon juice or if vinegar produced more curds. Background Information Curds were formed by a change in pH or by a change in temperature of the milk. Milk has a pH of 6.6 approximately‚ and at this pH the proteins were able to repulse each other‚ creating a negative charge. One of the proteins in milk is called casein‚ which when an acid of a lower pH than 6.6 was added‚ it will start to clump up causing curds to form and lactic acid to remain. In this reaction‚ the milk was serving as a base while
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PROTEIN ARTICLE RESEARCH SCI/241 PROTEIN ARTICLE RESEARCH Proteins are a part of every cell‚ tissue‚ and organ in our bodies. The protein we eat is broken down by amino acids that are later used to replace proteins in our bodies. These proteins include meats‚ poultry‚ fish‚ eggs‚ nuts‚ seeds‚ milk and milk products. Proteins are made up of amino acids. There are 20 different amino acids that join together to make up all types of protein. A complete protein source is one that provides all
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Fish phylogenetic tree based on protein size Amanda Reed Lab partners: Lecia Redwine‚ Kyle Hatcher TA: Baneshwar Singh Biology 117‚ Section 25 Tuesday 10:30 AM Introduction Although tree diagrams have been used since the days of Charles Darwin‚ biologists have only recently adopted the tree model of evolution to read and interpret phylogenies. One of the reasons for this is the confusion that often arises from using a tree model to describe a phylogeny (Baum‚ 2008a). Many people interpret
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Experiment 2: Estimation of protein concentration Name: Wong Jiun Hao ID: 00000011136 Cohort: BM114 Module: Biological Science Table of Contents Title 1 Table of Contents 2 Introduction 3 Objectives 4 Procedure 4 Results 5-6 Discussion 7-8 Conclusion 9 References 9 Introduction The concentration of protein in a sample can be obtained by using the Bradford protein assay method. The method requires a spectrophotometer
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