by and developed on physical agitation of the curd. Renault et al. (1997) found that syneresis could be influenced by changes in the protein conformation‚ essentially due to hydrophobic bounding. Many authors (Pearse et al. 1985; Stoll 1966; Walstra et al. 1985) indicated that whey protein denaturation syneresis rate can influence syneresis rate. Denatured whey protein rate is increased with increasing heat treatment temperature. The observed differences in syneresis can be related to the differences
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AP Biology 6th Mr.Scofield Blast Lab Background: Between 1990–2003‚ scientists working on an international research project known as the Human Genome Project were able to identify and map the 20‚000–25‚000 genes that define a human being. The project also successfully mapped the genomes of other species‚ including the fruit fly‚ mouse‚ and Escherichia coli. The location and complete sequence of the genes in each of these species are available for anyone in the world
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April 22‚ 2015 Does protein help to build muscle? The latest craze is about protein and how it affects building muscles and building muscle strength. Today‚ there are many athletes that use it as a supplement. There are many protein supplements available that are claiming to help people who work out to build muscle faster. Protein is a structure within our bodies that consists of amino acids. The cells in our bodies need this to function properly. If we did not have protein‚ our body’s functions
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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assays to detect the presence and absence of certain antigens or antibodies and also to quantify them when necessary. Quantification can be done in a range of microgram (µg) to nanogram (ng). The ELISA procedure takes advantage of the fact that most proteins will bind firmly to the surface of different kinds of plastic (polystyrene or polyvinyl chloride)‚ usually by hydrophobic interaction. The steps of the ELISA procedure are simple to perform. There are mainly FOUR phases in ELISA: 1. Coating
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Urinalysis Laszlo Vass‚ Ed.D. Version 42-0026-00-01 Lab RepoRt assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable
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Determination of Protein Content Using Kjedahl and Titration 2.0 Introduction Proteins are polymers. They are the source of dietary amino acids and are used for growth and maintenance of living systems. They are costlier sources of energy compared to carbohydrates and fats and hence the human body utilizes proteins mainly for biosynthesis rather than as an energy source‚ though the energy yield is 5 kcal/g of protein. Twenty different types of amino acids occur naturally in proteins. Proteins differ
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absorbing species‚ . When this information is graphed with absorbance on the y axis and at different points of concentration on the x axis‚ the best fit line equation will have a slope that is equal to Ԑ‚ giving us molar absorptivity (1). Then we can use the value of Ԑ to determine the concentration of [K2Cr2O7]‚ by taking the absorbance ratings and dividing them by Ԑ. Determining the order of reaction for a reactant‚ in this case K2Cr2O7‚ must be done experimentally (2). This is accomplished by making
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The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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Lab #4: The Immune System Purpose: The purpose of this lab was to perform and understand the procedures of conducting an ELISA test to determine whether a particular antibody is present in a patient’s blood sample through a virtual simulation. Hypothesis: If I successfully complete this lab‚ I will then understand how to perform an ELISA test‚ the purpose an ELISA test‚ and also how to interpret the results of this test. Materials and Procedures: Materials: Howard Huges medical
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