Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
Premium Transformation Trigraph Escherichia coli
Research Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually
Premium Enzyme PH Buffer solution
Determination of presence of protein in a variety of food substances by the use of Biuret Reagent Biol. 1040 Section 1005 Introduction The human body consumes carbohydrates‚ lipids‚ and proteins each day by eating a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular
Premium Protein Amino acid
Page 1/3 09/2010 SE 110.22 Forces in an Overdeterminate Truss The picture shows SE 110.22 in a frame similar to SE 112. * Comparison of forces in statically determinate and overdeterminate trusses1 * Bars with strain gauge full bridges to measure bar force1 * Computerised evaluation of experiments Technical Description Overdeterminate trusses are employed where overdimensioning is purposely required because safety must be maintained in the event of failure of an element‚ such as in aircraft
Premium Beam Force Truss
Purpose To check whether mass is gained or lost during a Chemical reaction. Hypothesis Equipments and Materials • Eye protection • Test tube • 250 ml Erlenmeyer flask and stopper • Weight Balance • Antacid tablet • Dilute solutions of o Sodium Hydroxide‚ NaOH(aq) o Iron(III) Nitrate‚ Fe(NO3)3 • Funnel • Cylinder Procedures 1. Put eye protection on for safety purposes. Part A: Reaction between Iron(III) Nitrate and Sodium Hydroxide. 2. Take two cylinders and fill one (full)
Free Sodium hydroxide Chemistry Sodium
original state and the percent of the hydrate recovered was calculated by using the mass of the rehydrated sample by the mass of the original hydrate and then multiplied by 100%. Data Presentation & Analysis Table 1: The data was collected from the lab experiment. Sample calculations are shown. Mass of beaker with sample 30.765g Mass of empty beaker 30.263g Mass of sample .502g Mass of beaker with sample after 1st heat 30.661g Mass of beaker with sample after 2nd heat 30.657g Heating mass
Premium Mathematics Mole Heat
Experiment #2 “Density Determinations” Report The objective for experiment #2 was “to determine densities of objects/salt solutions with different concentrations of salt‚ to see how density changes as a function of concentration.” In experiment #2‚ part II‚ calculations of Density of NaCL solutions were made from 0%-25% NaCL concentration. My hypothesis was that as the % increased‚ so would the density‚ because adding weight would increase the density of each solution. The density calculations in
Premium Measurement Density Standard deviation
Behavior of Gases and Absolute Zero Determination Lab Report Experiment 4 Introduction In this experiment we measured the relationship between temperature‚ volume‚ and pressure. Objectives: Conduct a set of experiments‚ each of which illustrates a gas law. Gather data to identify the gas law described by each activity. Complete the calculations necessary to evaluate the gas law in each activity. From your results‚ derive a single mathematical relationship that relates pressure‚ volume‚ and
Premium Pressure Temperature Gas
to help regulate how much water is taken and released along with the other chemicals involved. Stomatal density is the number of stomata per unit area of a leaf. The experiment conducted with leaves along a water source and away from a water source was to analyze the stomatal density difference. This experiment was performed to measure which environment had a greater effect on stomatal density. Stomata of a leaf is a miniature pore in the epidermis of a leaf surrounded by guard cells that have a
Premium Photosynthesis Water Plant physiology
Second (s) 2. Give the correct SI derived units of the following quantities. Density Speed Acceleration Force Pressure kg/m3 Energy 3. Convert the following: μm (Ans. 1.22) a. 1.22 x 10-9 km = b. 6.523 x 10-4 nm = c. 2.5x 10-9 nm3 = d. 30.5 cm/s = e. 9.6 ng/mm2 = 4. Mm (Ans. 6.523 x 10-19) mm3 (Ans. 2.5 x 10-27) km/h (Ans. 1.09 x 106) kg/m2 (Ans. 9.6 x 10-6) Perform the following calculations and report each answer with the correct number of significant figures and units. b. 2.457
Premium Kilogram Gold Mass