Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
Premium Glycerol Glucose Liver
EXPERIMENT I Photoreduction of Benzophenone Introduction The study of chemical reactions‚ isomerizations and physical behavior that may occur under the influence of visible and/or ultraviolet light is called Photochemistry. The fundamental principles for understanding photochemical transformations are that light must be absorbed by a compound in order for a photochemical reaction to take place‚ and that for each photon of light absorbed by a chemical system only one molecule is activated for
Premium Oxygen Sunlight Electromagnetic radiation
Hannah Herbert BioL121 9/16/14 Density and Making Solutions Objective and Background/ Theory of Experiment: The objective of the experiment was to determine the density of a metal along with the density of distilled water. In an attempt to help the experimenters more thoroughly understand the relationship between concentration and density. Archimedes a Greek mathematician in the third century B.C. originally determined the relationship between the amount of matter that is within a particular space
Premium Density Concentration
CHEM 161L (603) 6/2/2014 Lab report 1: Density Purpose: The purpose of this lab was experimentally determining the familiarization between volumetric and gravimetric using common equipment. Procedure: Equipment: Objects for density determination – a metals‚ and Teflon ball 50 mL graduated cylinder 100 mL graduated cylinder 50 mL beaker Electronic balance Calcaulator Water Ammonium sulfate Step: Part 1.Measurment of the density of a solid 1. Three metal objects
Premium Water Volume Liquid
Radius of shaft G = Shear modulus [pic] = Angle of twist L = Length of shaft As torsional stiffness [pic]‚ it can be determined through [pic] ………….. ( Equation 5 ) Apparatus : • One solid circular disk with mass = 4.536kg‚ diameter = 150mm and thickness = 30mm. • One annular circular disk with mass 1.89kg‚ outer diameter 150mm‚ inner diameter = 110mm and thickness = 30mm. • Two chucks; one steel rod; one stopwatch
Premium Torque Inertia Fundamental physics concepts
Wave Nature of Light Objective: The purpose of this lab is to investigate interference‚ otherwise known as the diffraction of light. A beam of light acts a wave‚ and we are able to use equations so calculate the wavelength of the light used. The diffraction of a straight edge demonstrates that light waves bend around straight edges‚ allowing light to enter an area of shadow. When waves are superposed‚ they reinforce each other when crests are in phase and cancel out when they are not in phase
Premium Diffraction Wavelength Light
LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
Premium Plasmid Transformation Escherichia coli
PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
Premium Oxygen Carbon dioxide Ratio
was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane. The polar absorbent material in the column separated the different pigments by allowing the least polar molecules to travel through the column faster than the more polar molecules
Free Solvent Acetic acid Ethanol
Chemical Report The chemical I will be writing this report on is oxygen. The name oxygen comes from the Greek word “oxygens‚” which means “acid producer”. Dictionary.com defines oxygen as “a colorless‚ odorless‚ gaseous element constituting about one-fifth of the volume of the atmosphere and present in a combined state in nature.” Oxygen has many uses today. First‚ oxygen is used by people‚ plants‚ and animals in the respiration process. Tanks of oxygen are used in the healthcare field to treat
Premium Oxygen