Three-Dimensional Structure of Proteins Rotation around the -Carbon in a Polypeptide A Sterically Nonallowed Conformation The Helix and Pleated Sheet Conformationally allowable structures where backbone is optimally Hbonded (linear Hbonds). Helix (3.613 Helix): •3.6 residues/turn •Rise = 0.15 nm/ residue •13-atom hydrogenbonded loop Linus Pauling and Robert Corey‚ 1950 Pleated Sheet: •Anti-parallel or parallel •2.0 residues/”turn” •0.34 nm/residue (anti-parallel) or 0.32 nm/residue
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How Credit Cards Work Have you ever stood behind someone in line at the store and watched him shuffle through a stack of what must be at least 10 credit cards? Consumers with this many cards are still in the minority‚ but experts say that the majority of U.S. citizens have at least one credit card -- and usually two or three. It’s true that credit cards have become important sources of identification -- if you want to rent a car‚ for example‚ you really need a major credit card. And used wisely
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Title: Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method
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institutions. As a consequence‚ the necessary attention for the global challenges fade into the background. This essay attempts to establish how both global
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Introduction to How Gas Prices Work Image Gallery: Hybrid Cars Photo by Justin Sullivan/Getty Images High gas prices can make you stop and think about your commute. See hybrid car pictures to see models that save you money.In May 2008‚ average gas prices in the United States approached‚ and in some places passed‚ $4.00 a gallon‚ shattering records. But this was nothing new to American consumers. May was a month of records that broke one after another‚ and that came on the heels of months of
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Elise Oswald Instructor Richard Turner November 10‚ 2013 How to Make Elise’s Favorite Omelet There are many different types of omelets in the world but no one can make an omelet quite like Elise. When Elise makes her favorite omelet‚ there are many steps and each one is done with the upmost love and care. Elise always follows the same procedures and takes her time to form her perfection. Unlike most omelets her omelet is one of a kind because of the careful attention that is put into every
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Abstract There are many methods employed to precipitate proteins out of solution. In this experiment we manipulated many physical and chemical variables in order to achieve purification of a protein via precipitation. In the first part of the experiment we purified the protein casein by modifying it’s pH. In the second part of the experiment we manipulated the ionic strength of albumin in egg whites‚ in a process called salting out. By manipulating these chemical properties we were able to
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The first half of module seven discussed protein digestion‚ absorption‚ and metabolism; while the second half focused on pathological stress and kidney disease. Protein digestion occurs in the stomach and small intestine. When a protein reaches the stomach it is denatured by hydrochloric acid. Once the protein is digested pepsin breaks apart the denatured protein into peptides. Peptides move into the small intestine where they encounter an enzyme that digest the peptides into di- or tripeptides‚
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[107.1] understand how to make telephone calls 1.1 Describe the different features of telephone systems and how to use them Telephones have many Different features that a business can use to their advantage One of these features is telecommunications. This enables users to have conversations‚ in a business‚ this would be used to communicate within the business‚ and also make external calls to clients/customers. Another feature is “Call Holding”. This enables the user to put the call on
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beginning of the semster. This manual is expected to cover the safety‚ instruction and details of the experimental work during the lab sessions. All students should read the experiment before coming to the laboratory. The teaching assistant will discuss the safety precautions‚ experimental procedures and techniques involved in the lab session. The students should conduct the experimental work under supervision of the instructor and the TAs then a lab report (see later the required format) covering
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