upstream and downstream fragments‚ 200 ng of pyrG marker fragment and adjust to 50 μL of double-distilled water. 35. Lysis Buffer: to prepare 50 mL of buffer dissolve 23.6 g of Guanidine thiocyanate (118.16 g/L) in 25 mL of double-distilled water. Once dissolved add: 2.5 mL of 1 M Tris-HCl pH 7.0 (121.14 g/L)‚ 2 mL of 0.5 M EDTA pH 8.0 (186.12 g/L) and 0.05 mL of Triton X-100. Add double-distilled water to 50mL (final concentrations: 4 M guanidine thiocyanate‚ 50 mM Tris-Hcl pH 7.0‚ 20 mM EDTA and 0.1
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Alisha Prakash Biol 111-513 The Properties of Water; Osmosis and Diffusion Hypothesis: When animal and plant cells are placed in a solution‚ the movement of water is effected by the solute concentration of the solution. If animal and plant cells are placed in a more concentrated solution‚ the solution becomes hypertonic to the cells‚ causing water to exit the cells. This changes the appearance of the cells‚ causing the cells to look darker (more concentrated) and shriveled up. Null Hypothesis:
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11.1 Washing with cold distilled water Washing process is not only remove soluble protein‚ fat‚ and pro-oxidant‚ but may also remove antioxidant. Therefore the oxidative stability of muscle ultimately depends upon the antioxidant/prooxidant balance that remains after the muscle is washed. It suggests that washing may promote oxidation in mince since it effectively removes antioxidants from the fish. In addition‚ the neutral lipids are more easily removed than polar lipids‚ phospholipids and free
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Blast Off! Lab # 4 Purpose: To design an experiment to test several variables that affect the build up of gas in the closed canister. Hypothesis: I think that we will have to adjust the amount of water to control the speed of the reaction. Materials: · Apron · Safety Goggles · 4 Alka-Seltzer Tablets · 2 Plastic Film Containers (with lids) · Water · Stop Watch or Timer · Graduated Cylinder Procedure: Step 1. Put on apron and Safety goggles. Step 2. Get Alka-Seltzer tablets and plastic film
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The inverted water method MLA citation BBC News. BBC‚ n.d. Web. 09 Nov. 2015. "Factors Affecting Enzyme Activity." Factors Affecting Enzyme Activity. N.p.‚ n.d. Web. 14 Nov. 2015. Enzymes. Scenario experiment AIM: To investigate one factor that affect the distribution of hydrogen peroxide by the catalase in this organism. In this catalase and hydrogen peroxide experiment‚ we will find out how enzymes act as catalysts by causing chemical reactions to occur. Using a potato and hydrogen peroxide
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Using a cooling glass of water to determine the area and volume of the curve formed. Research Question: To what extent can a cooling glass of water be modelled accurately and be used to find the area under the curve and volume formed by curve? Data Collection: In order to produce an exponentially decaying graph‚ I had to first heat a glass of water for a certain period of time {150 ml of water}. After the water was heated a temperature probe‚ connected to logger pro and laptop
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Purpose of lab 1.4 Measuring Volume by Displacement of Water was to determine the fraction of air in different granulated solids. The lab cocised of one test done on four different granular solid; with up to 3 trials on each solid. When all tests are done and put in tables‚ and the calculations are made‚ the conclusion is that grain size does not affect that fraction of air. To be able to find the fraction of air‚ 3 measurement had to be taken‚ the volume of the solid with air‚ the volume of water‚ and
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What organic molecule can be detected with Lugol’s? By using the Lugol’s iodine test‚ we can detect the presence of starches. Why put Lugol’s in the beaker and not the bag? When we put the Lugol’s regent (I2Kl) in the beaker‚ we can observe the effects of osmosis as the Lugol’s regent (I2Kl) solution on the outside of the beaker permeates the intestine (Dialysis tubing) inducing a chemical reaction that will turn the liquid in the intestine (Dialysis tubing) blue-black. If the amylase successfully
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LAB 5: Coliform bacteria in surface waters I. Introduction Many organisms‚ including humans‚ have symbiotic bacteria in their guts that aid digestion. Symbiosis is an intimate relationship between different organisms in which both the host organism‚ e.g. the human‚ and the symbiote‚ e.g. bacteria‚ benefit from each other. In this case‚ the bacterium gets a favorable environment and food source in the intestines of a human. In return‚ these bacteria improve the digestibility of food through
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Reactions’ lab is to learn how to plan an investigative lab without outside help and to learn about the ‘Rates of Reactions’. This lab will require students to take responsibility for their own learning and use their prior knowledge to aid in the completion of this lab. In the lab prepared below‚ students will be learning about Rates of Reaction with varying concentrations of Alka-Seltzer with water. Hypothesis: I believe that the more water added to the canister with the Alka-Seltzer tablet‚ the
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