Practical 5: determination of activation energy of an enzymed catalyzed reaction Introduction: enzymes are complex chemicals that control reactions in living cells. They are biochemical catalysts‚ speeding up reactions that would occur too slowly to be of any usefulness to an organism. Although in organisms‚ it is not necessary for the reactions to be at maximum rate at all times. Enzymes interact with other molecules to produce a stable system in which the products are made when they are
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Purpose: The purpose of this lab was to see how the changes in exercise intensity affect the rate of metabolism within the body. Methods: In this lab‚ indirect calorimetry was used to measure metabolic rate by calculating caloric expenditure by the measurements of oxygen consumption. The variables measured were the fraction of oxygen expired‚ the fraction of carbon dioxide expired‚ and the total volume of air inspired. This experiment required the participation of two volunteers. First‚ the variables
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Mallory Charland February 9‚ 2015 Mutations of E. coli Lab Report Advanced Biology Deducing Mutations of the Lac Operon of E. coli Abstract: In this lab we determined a possible mutation in unique bacterial strains of E. coli by observing the proper responses of wild type E. coli Lac Operon as a control group. Mutated strains of E. coli were placed in four test tubes‚ each containing a different substance (lactose‚ glucose‚ water and lactose and glucose)
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Life Science Lab. A. Arnold Tuesdays @ 2:30 September 30th‚ 2011 Lab Report: Nuclear and Cell Division. PART A: Stages of Mitosis in my own words. 1. Interphase: DNA has formed already‚ but it remains in the simple form of chromatin. Chromatins are structures that are loosely coiled in the cell.3 I also observed during my lab that this was the only stage where I could still see a nucleus and nucleolus intact within the cell; this is because it’s the only stage where the nuclear membrane has
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Determination of citrinin produced by P. notatum and A. nigerin liquid media The filtrates obtained from above experiment were used for extraction and estimation of citrinin. Briefly‚ the citrinin was extracted thrice with chloroform (1:1 v/v). All the three extractions were pooled and concentrated in vacuum at 40°C using a rotary evaporator. The crude extract was then diluted in minimum amount of chloroform (2ml) and citrinin was estimated by thin layer chromatography (TLC) according to Razak et
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OBSERVATIONS Observations The "enzyme" we chose was Mohammed‚ a boy. The table we worked on was flat and smooth. Data Tables and Graphs *the graphs are separately attached. Control- Enzyme Reaction Time (10 seconds) Number of Reactions Reaction Rate 10 2 2tp/10
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respiratory exchange rate‚ or RER. An RER of 0.7 means that carbohydrates are being used for fuel‚ and a ratio of 1.0 means that only fatty acids are being used for fuel; of course‚ values can fall between. Methods: To observe the effects of prolonged exercise on the metabolic system the lab subject needed to walk on the treadmill at a rate that kept his heart rate at 60% of his maximum. The
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Lab report for Experiment #2: Extraction Your Name: Name of TA: Lab Partner’s Name: Lab Section: Title: Experiment #2: Extraction Purpose: What is the purpose of this lab? In your OWN words! Observations: Weighed out 3.2568 grams of chemical mixture that was yellow in color. Dissolved dry chemicals in 38 ml CH2Cl2 with gentle heating. Poured the yellow solution into sep funnel. Added 10 ml CH2Cl2 to flask to rinse‚ poured solution into sep funnel. Added 15 ml 3 M
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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go through the whole process of osmosis. Also‚ a lot of the experiment was rushed as we were under pressure to finish as quickly as possible. Unfortunately the time constraint was caused by and outside influence unrelated to the lab itself‚ and so next time we could do a lab under normal conditions where there are no outside disturbances. There were weight differences in the potatoes which could have caused thicker potatoes to absorb more than others. It was a little impossible to obtain the same
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