Vitamin c in fruit juice using dcpip titration Preparing the standard ascorbic acid solution 1. Weigh out accurately about 0.2 g ascorbic acid and make up to 1 L with distilled water. 2. Calculate the concentration of the ascorbic acid solution: C = n/V = m/M/V. Sample calculation: mass of ascorbic acid = 0.205 g C (ascorbic acid) = 0.205/176.12/1.00 = 0.00116 M Preparing the DCPIP solution Weigh out accurately approximately 0.24 g DCPIP (Mr = 268.1g/mol) and make up to 1 L with
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Acid Determination of Vitamin C Tablets and Salt Solutions Chem 1290-016 03-05-2010 ------------------------------------------------- Purpose: The purpose of this lab was to use acid-base titrations to find the mass percent of ascorbic acid in a Vitamin C tablet. A known concentration of sodium hydroxide was used and the value found was compared to the stated amount of ascorbic acid listed on the vitamin container. Also in this lab the acidity of different salt solutions were tested using
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amount of vitamin C in fruit juices affects its freshness and till what extent is the information provided on the pack by manufacturers is reliable? Background Information: Hypothesis: -fresh juices have more vitamin C than long-life -juice that is ‘not from concentrate’ is best in terms of vitamin content -if heat destroys vitamin C then heat-treated long-life juices will have lower concentrations -if heat destroys vitamin C then boiled fruit juice will have lower concentrations than unboiled
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investigate the enzyme concentration and rate of reaction Background To carry out our experiment we used 3 different types of orange juices‚ and we also used 0.1% of vitamin C solution. The aim of our experiment was to see how much DCPIP was needed to be added to make the orange juices turn back to the orange colour and the DCPIP to decolourise once the 0.1% of vitamin C was added. DCPIP is a blue dye in its non-reduced form; it becomes colourless when it gains electrons. Vitamin C is an anti – oxidant
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differences of percentage of vitamin C (ascorbic acid ) between the fresh orange juice and artificial orange juice (Sunkist). Problem statement : Does have different types of fruit juice contain similar amount of vitamin c ? Objevtive: To analyse the concentration and percentage of different food sample by using titration method and state whether the percentage and concentration of vitamin C. Methodology: Theory : DCPIP can also be used as an indicator for Vitamin C. If vitamin C‚ which is a good reducing
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DETERMINATION OF VITAMIN C CONTENT IN COMMERCIAL (CENOVIS) TABLET ABSTRACT In this experiment‚ the vitamin C content of a commercial tablet is determined and compared with the manufacturers’ specification. Vitamin C is water-soluble and is an enantiomer of ascorbic acid. (Commercial vitamin C is often a mixture of ascorbic acid and other ascorbates.) Ascorbic acid‚ C6H8O6‚ is a reducing agent that reacts rapidly with iodine (I2). As the iodine is added during the titration‚ the ascorbic acid is
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Vitamin C –A Natural Antioxidant Concept: Vitamin C (ascorbic acid) is an antioxidant. The amount of vitamin C content in food can be found out by titrating with an oxidising agent‚ e.g. iodine. In the reaction‚ vitamin C is oxidised‚ while iodine is reduced to iodide ions. The endpoint is determined by the formation of blue-black starch-iodine complex when all vitamin C is oxidised and excess iodine is free to react with the starch solution added as indicator. Introduction Vitamin C is an
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Winny Stephanie Experiment 1: Quantitative Determination of Protein Concentration Using the Biuret Test Table 1: Experimental protocol for construction of the protein standard curve Tube 1 2 3 4 5 6 Buffer (ml) 1.0 0.8 0.6 0.4 0.2 0.0 BSA Protein solution (10 mg ml-1) (ml) 0.0 0.2 0.4 0.6 0.8 1.0 Biuret reagent (ml) 4.0 4.0 4.0 4.0 4.0 4.0 Total Volume (ml) 5.0 5.0 5.0 5.0 5.0 5.0 Final protein concentration (mg ml-1) 0 2 4 6 8 10 Absorbance 0.000 0.092 0.163 0.272 0.363 0.474 Table
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E20-1 Experiment 20 Consumer Chemistry: Vitamin C in Fruit Juices The Task The goal of this experiment is to determine the concentration of vitamin C in a range of different fruit juices (fresh and preserved) using titration and to rank these sources of vitamin C. Skills At the end of the laboratory session you should be able to: * use a pipette correctly‚ * use a pipette filler safely‚ * manipulate a burette and carry out a quantitative titration properly‚ * weigh a sample
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experiment 1 Analysis of Commercial Vitamin C Tablets Objective To employ titration technique to determine the content of vitamin C in commercial tablets using volumetric analysis and compare it with the manufacturers’ specifications. Introduction Deficiency of essential antioxidant Vitamin C in human will lead to scurvy. In this experiment‚ the vitamin C content of commercial tablets is determined and then compared with the manufacturers’ specifications. Vitamin C‚ ascorbic acid C6H8O6‚ reacts
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