the enzyme concentration is decreased then the rate of reaction will decrease. Controlled: temperature Independent: % catalyze concentration ( Dependent: rate of reaction (mm/s) Materials: Mortar and pestle Graduated cylinder Distilled water Test tubes Chicken liver (or any other liver) Sterile transfer pipet (Dropper) Sterile weigh boat Hydrogen peroxide Razor blade Cutting board Electronic balance Timer Ruler Clean everything Calculate mass of chicken liver needed for
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Synthesis of Butyl Benzoate Using Phase Transfer Catalysis The objective of the experiment is to synthesize the butly benzoate by nucleophilic substitution and characterize it by IR spectroscopy. The percent yield of the final product is determined after the synthesis. Procedures: 2.0 mL of 1-bromobutane‚ 3.0 g of sodium benzoate‚ 5.0 mL of water‚ 4 drops of Aliquat 336‚ and a boiling stone were placed in a 50mL round-bottomed flask. The reaction mixture was refluxed for 1 hour and the flask
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Experiment 3: ANALYSIS OF UNKNOWN ACID SAMPLE USING TITRATION METHOD Date of Experiment: 4 September 2012 Introduction An acid-base titration is a procedure used in quantitative chemical analysis to determine the concentration of either an acid or a base. Titration is the slow addition of an acid (or a base) of known concentration from a burette (a narrow graduated cylinder) to a base (or an acid) of unknown concentration fin an Erlenmeyer
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Lab 17 Amino Acids and Proteins Lab date 10/22/2013 12-1350 I Purpose The purpose of this experiment was to separate mixtures of II Method For a complete list of experimental procedures see prelab outline attachment #1. “For a complete list of experimental procedure see Seager‚ Spencer L. and Slabaugh‚ Michael R. Safety-Scale Laboratory Experiments for Chemistry for Today General‚ Organic and Biochemistry; Thomson Brooks/Cole‚ Belmont‚ CA‚ USA‚ 2008; pp.221-225”. III Data Part A Mass
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indicate the end point of a titration) titrations as it forms stable complexes with most metal ions. EDTA is a tetracorboxylic acid and in alkaline conditions‚ it exists as Y⁴⁻ ions‚ which form 1:1 complexes with metal ions like nickel(II) ions: Y⁴⁻ + Ni²⁺ NiY²⁻ A metal ion indicator (an organic dye which changes colour when it binds with metal ions) shows the end of an EDTA complexometric titration. However‚ for a metal ion indicator to be suitable in n EDTA titration‚ it must not bind as strongly
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Abstract: This report presents the reason why the ocean does not freeze in the winter. Since the ocean consists of salt water‚ an experiment was done to test the effect of freezing on salt water compared to pure water. The experiment was conducted three times in order to obtain accurate results and eliminate errors. In addition to the salt and pure water experiment‚ a variation of this experiment was created to test other options. In the variation experiment the effect of freezing on sugar water was conducted
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[pic] Department of Chemical & Biomolecular Engineering THE NATIONAL UNIVERSITY Of SINGAPORE Chemical Engineering Process Laboratory I SEMESTER 4 Experiment F2 Flow Measurement in Closed Conduit and Centrifugal Pump Characteristics Name : Ang Sok Gek Chai Chang Er Cherry Chen Mingli Matriculation No. : U046941L U046938W U046882J Group : Th1 Date of Experiment : 16th March 2006 Table of Contents
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commercial vinegar using titration method. Chemical reaction involved: CH3COOH(aq) + NaOH(aq)→ NaCH3COO(aq) + H2O(l) Apparatus: An electronic balance‚ a 10.0cm3 pipette‚ a 25.0cm3 pipette‚ a pipette filler‚ a 250.0cm3 volumetric flask‚ a burette‚ stand and clamp‚ a conical flask‚ a white tile‚ 3 large beakers‚ a small beaker‚ a dropper‚ a boiling tube‚ a rinsing bottle Chemicals: 0.1M NaOH(aq)‚phenolphthalein indicator‚ commercial vinegar (5% acidity)‚ distilled water Procedures: Part1:
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Chemistry Experiment Double Indicator Titration 1 Aim The purpose of this experiment is to find the composition of a sample of sodium carbonate mixture by titration. Introduction The reaction between sodium carbonate and hydrochloric acid occurs in 2 stages‚ with the formation of hydrogen carbonate ion as the intermediate product. Na2CO3(aq) + HCl [pic] NaHCO3(aq) + NaCl(aq) NaHCO3(aq) + HCl(aq) [pic] NaCl(aq) + H2O(l) + CO2(g) [pic] Requirements Hydrochloric
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Enzyme Lab Using Jello INTRODUCTION: Enzymes are known as protein catalysts. The name protein catalyst suggests that most enzymes are made of proteins. A catalyst is a substance that speeds up chemical reactions without being consumed in the process. (Giuseppe‚ M 2002‚ p.69). After a reaction has been catalyzed‚ the catalyst can be used again to catalyze the same reaction. Enzymes reduce the activation energy (minimal energy) it takes for a reaction to take place. Enzymes can either catabolize
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