Sampling of Gram-Positive Bacteria and Antibiotic Resistance Resting We thought it would be interesting to find out the different bacteria that grow on things we use on a daily basis and the level of antibiotic resistance that those bacteria have. We chose to swab the toilet seat in the MATC women’s bathroom and the ATM machine in the commons area on campus. We were certain the ATM would be dirtier than the toilet seat since the bathrooms get cleaned every day. The first step we took in testing
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Investigation of the probiotic properties of bacterial strains from two probiotic drinks and their survivability in artificial gastric juice ABSTRACT: Two probiotic drinks were investigated in vitro to test their ability to survive acidic conditions and their probiotic factors. Both the products: Actimel and Yakult contain gram-positive bacteria‚ but Actimel also has a gram-negative bacteria. The ability to survive was investigated by adding artificial gastric juice to the products and incubating
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Autumn White Biology 258-05 November 26‚ 2012 Unknown #19 Enterbacteriaceae Enterobacter aerogenes ABSTRACT The objective of this report was to identify an unknown microorganism through several differential media tests. Over the course of a couple weeks‚ ten tests were performed. First‚ a gram stain was performed‚ indicating the bacterium was gram negative. An aerotolerance test determined that the bacterium was a facultative anaerobe. Next‚ a negative result in the
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Michele Hindmarsh mhindma@my.wgu.edu Student ID# 000383032 MLT1 – Experiment 5; Task 6 Differential Staining Heidi Atkinson‚ MS Lab Experiment #5-Differential Staining Through the process of differential staining‚ there are distinct differences between the cell walls of gram-positive and gram-negative bacteria. In the case of gram-positive bacteria‚ the cell wall is comprised of 60-90% peptidoglycan and is very thick. There are numerous layers of teichoic acid bound with peptidoglycan
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Abstract Three tests were performed to determine the identity of unknown number 34‚ a gram stain‚ citrate utilization test‚ and a urease detection test. These tests are explained in detail‚ and the method of performing the tests is described. The tests showed that the bacterium was gram-negative bacilli that did not utilize citrate‚ and the ability to hydrolyze urea using urease. These results indicate that the identity of unknown number 34 is Proteus mirabilis. Introduction Proteus mirabilis is
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and Staphylococcus spp are commonly opaque white colonies when grown on TSA (Bergey). Additionally‚ Ex 3-10 Endospore Stain showed the EI to be non-endospore forming‚ which corresponds to the characteristics listed in Bergey s Manual of Determinative Bacteriology under the species Staphylococcus (Bergey 464). Ex 5-28 Motility Test showed the EI to be non-motile‚ which is also a key characteristic of Staphylococcus species
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Works Cited Bergey‚ D. H.‚ J. G. Holt‚ et al‚ et al. Berge ’ys Manual of Determinative Bacteriology. 9. Philadelphia‚ PA: Lippincott Williams & Wilkins‚ 1994. 528-549.
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Proteus mirabilis The genus Proteus‚ according to Bergey’s Manual of Determinative Bacteriology‚ is a group of straight rod Gram negative bacteria around 0.4-0.8 μm in diameter and 1-3 μm in length. In the microscopic test done‚ it was seen that the unknown culture contained rods that are on the shorter side‚ thus was identified as bacteria that appear to be coccobacilli. Because of the presence of peritrichous flagella‚ species included this genus are motile and most strains swarm in periodic cycles
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References: 1. Bergey DH‚ Holt JG. Bergey ’s manual of determinative bacteriology. 9th ed. Philadelphia‚ PA: Lippincott Williams & Wilkins; 1994:177. 2. Whalen‚ J. G.; Mully‚ T. W.; English‚ J. C. (2007). "Spontaneous Citrobacter freundii Infection in an Immunocompetent Patient". Archives of Dermatology 143 (1): 124–125
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Phelps‚ Biology 205 MW 2:10pm. Unknown Organism Paper The unknown organism tested in the Mesa lab was collected from in between the toes of my puppy‚ Riley. The original culture grown consisted of dozens of visibly different organisms of varying colony colors and growth patterns. I chose to test a smaller‚ red colony for my experiments. Based on its gram reaction and oxygen requirements‚ the following tests were performed to reach my presumptive ID: Test Performed Result Gram Stain +
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