The concentration of 1-kestose isomer increased steadily with time from 0 - 80 min. During this time‚ sucrose was significantly influencing the rate of 1-kestose formation and hence the optimum concentration of the 1-kestose isomer was observed at 80 min. After 80 min‚ the 1-kestose concentration gradually decreased. This was because the invertase active sites had become saturated with sucrose (substrate) or sucrose had been used up in the reaction. Therefore‚ there was a gradual decrease observed
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Service - Our Responsibility Water and Sewerage Authority (WASA) was established by an Act of Parliament in 1965 to manage the water and sewerage sector of Trinidad and Tobago. An essential component of this mandate is the delivery of a safe‚ reliable and efficient water supply to satisfy the demand of all sectors of the economy. Whilst over the ensuing years there has been a steady increase in demand‚ there has not been the commensurate investment in infrastructure and the identification of alternative
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performed was the fermentation of sucrose and arabinose. This was performed by aseptically inoculating a tube of phenol red sucrose broth‚ and a tube of phenol red arabinose broth with the unknown culture and incubating at 37 degrees Celsius for 48 hours. After incubation‚ the two tubes were examined for color change. The eighth test was to find out if the bacteria in question had flagella. The motility test was performed by aseptically inoculating the unknown bacteria into a tube of TSA broth
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Microbiology Unknown 5/7/2013 Microbiology Unknown #94 The purpose of this report was to identify an unknown bacterium by applying methods that were previously learned and conducted in microbiology lab class. It is possible to identify a mixed culture by running experiments on the unknown bacteria and taking what we already know and applying it to the results. It helps to slowly eliminate any bacteria that do not correspond with the results of certain tests. An unknown test tube labeled 94 was
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"Do different concentrations of solutions determine the mass of a potato?" Introduction: The way to get the full results of this lab was through the process of osmosis. Osmosis is the movement of water across a membrane into a more concentrated solution to reach an equilibrium. When regarding cells osmosis has three different terms that are used to describe their concentration. The first of these words is isotonic. Cells in an isotonic solution show that the water has no net movement and the amount
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21 Recommendations 21 References 22 Appendices 22 Abstract This experiment was performed in order to determine the molarity of a solution and the percent by mass of acetic acid‚ CH3COOH in vinegar. Acetic acid is monoprotic and belongs to the carboxyl family of organic compounds. The titration method is used to neutralize the acids by using the standardized sodium hydroxide solution. It reacts with bases which is sodium hydroxide‚ NaOH to form salt and water. The equivalence point of this
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article 1. the The purpose of this is knowing the types of bacteria that exist in our environment and diseases caused by them‚ and how treat it. MATERIAL AND METHODS: An unknown bacterium was handed out
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converted to sugars through a process that is known as fermentation. Sugars in the form of sucrose are mostly used by humans (Alexander N.Glazer). Sugar cane and sugar beet are composed of 20% sucrose‚ 75% water‚ 5% cellulose and about 1% is inorganic salts (Alexander N.Glazer). Sucrose is extracted with water by using mechanical pressure from the sugarcane. On the other hard the beet is peeled and crushed. Sucrose that was extracted from sugarcane is a highly effective substrate that is used during
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Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons‚ such as knowing the causative agent of disease‚ knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study
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The purpose of this report is to explain the process and steps used to identify a certain microorganism referred to as the unknown. Materials and Methods A test tube with an unknown microorganism will be retrieved. Once the test tube is retrieved‚ a steak for isolation will be completed in order to produce isolated colonies of the organism on an agar plate. The unknown test tube with the bacteria is flame sterilized using a Bunsen burner. Once the bacterium test tube has been flame sterilized
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