measured 10g of ascorbic acid powder and mix it within 1 litre of water. I believed that by having the solution prepared before putting in the d-block elements would save time overall. Unfortunately‚ the data was all over the place‚ this is because the later I conduct the experiment‚ there is a higher chance of oxidation occur in the ascorbic acid solution as it have a direct contact with oxygen in the atmosphere. In order to keep constancy‚ I had to reduce the amount of ascorbic acid by ratio to 0
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experiment. To each of the Petri dishes 1 mL of buffer solution was added. At station 1 the control two drops of ATP were added to each Petri dish. The control Petri dish was left alone and a pipette was used to deliver several drops of acetic acid to the experimental dish. Several drops of sodium hydroxide were added to the experimental dish. Results were observed and recorded. At station 2 the control Petri dish was left alone on the table
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Introduction In the amylase lab‚ it was being tested if amylase‚ an enzyme found in saliva‚ would be denatured by being put in an acid or high temperatures. This lab is about denaturing amylase. It is tested by exposing it to pH and temperature changes. It is then mixed with Benedict’s solution‚ is a solution that changes color from blue to reddish brown when maltose is present. Amylase breaks starch into maltose‚ so is the amylase isn’t denatured‚ it should change colors. Amylase is an enzyme
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50- 500 μg/ml‚( each of these concentration was taken in duplicate) was mixed with 1800 μl of ABTS solution was mixed with it for each concentration. Similar process was repeated for ascorbic acid concentration. All test tubes (ethyl acetate soluble fraction‚ ethyl-3-hydroxy-5-methoxy-4-methylbenzoate‚ ascorbic acid‚) labeled separately were shaken well and incubated in the dark at 25oC for 30 minutes. Then the Kinetic absorbance was recorded at 734 nm by spectrophotometer after 1 and 6 minutes for
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supports the general growth-enhancing effects of the substance‚ but does not conclusively demonstrate its mechanism. Unfortunately‚ the third experiment in which acrylic acid was used to inhibit the β-oxidation pathway did not clarify the origin of this effect. The absence of difference between growth of samples with acrylic acid‚ with and without squalane is ambiguous in its interpretation. This may mean that the
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Enzyme lab report. Introduction. An enzyme is a protein molecule that speeds up the rates of chemical reactions by many folds. They recognize‚ bind‚ and change specific reactants. They do not change thus can catalyze the same reaction again and again. Activation energy also known as an energy barrier is the amount of energy needed in order to begin a chemical reaction. Catecholase catalyzes the reaction rate of catechol oxidation. Catechol is found beneath the skin of many plants such
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Unknown Lab Report #1 Unknown #1 April 25‚ 2012 Microbiology Spring 2012 MCB2010C Unknown #1 Introduction Identity of a microorganism has proven to be very significant. Doing so can help identify diseases and created treatment and cures for such diseases. As a result‚ various laboratory tests were performed to an unknown microbe (Unknown #1) found in the water of a nearby pond. By identify the microbe‚ the safety of the water will be known to those around it. Materials and Methods
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called photosynthesis. This process is used by plants and other organisms for synthesis of lipids and amino acids or can be metabolized during cellular respiration to produce ATP. This process takes place in chloroplasts‚ which is a plastid that contains chlorophyll and involves two interlinked reactions‚ which are light dependent reactions and light independent reactions. Throughout our lab experiment‚ we focused on the affect access of light has on carbon dioxide during photosynthesis. Carbon dioxide
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The Effect of Concentration on Absorbance Background Information The purpose of the “Determining Solution ‘Concentration’ Using A Spectrophotometer” lab was to use a spectrophotometer to find the relationship of concentration and absorbance obeying the Beer-Lambert law‚ which states concentration and absorbance are directly related‚ to then further determine the concentration of three unknown solutions. With the assumption that the solutions obey the Beer-Lambert law it is predicted that as concentration
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In the unknown identification labs‚ we have identified our unknown as Pseudomonas aeruginosa. Pseudomonas aeruginosa is Gram negative and rod shaped that we found to be motile in the lab. Our strain of P. aeruginosa formed colonies that were round in shape and had scalloped margins on nutrient agar. On our agar slant‚ the P. aeruginosa colonies had a filiform appearance on the edges. I think we correctly identified our unknown as P. aeruginosa because we performed several different tests‚ eleven
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