"Determining optimum temperature and ph for enzyme reaction" Essays and Research Papers

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    Ph and Osmosis

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    The Effect of pH on the Rate of Osmosis Using a Glucose Solution Melissa Werderitch Biology 157 11/6/06 Introduction In a journal article written by Florian Lang‚ osmosis is essentially explained as the flow of water from one area to another that are separated by a selectively permeable membrane to equalize concentrations of particles in the two locations (Lang‚ 1997). Osmosis is able to maintain osmotic pressure and regulate a cell’s volume. In a hypotonic () or hypertonic () environment

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    Purpose: To find out the percent yield of copper in the reaction between copper sulfate (CuSO4) and Iron (Fe). Materials: Balance 100-mL beaker 250-mL beaker Bunsen burner Copper sulfate crystals Glass stirring rod 100-mL graduated cylinder Iron filings Ring stand and ring Wire gauze Procedure: 1. Record mass of clean 100-mL beaker. 2. Add 8.0 grams of copper sulfate crystals to beaker. 3. Add 50.0 milliliters of distilled water to the crystals. 4. Put wire gauze on ring on ring

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    Enzyme Lab

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    MISEP Chemistry 512 – Jacobs Enzyme Catalyst Lab - Formal Report – August 8‚ 2007 ABSTRACT This investigation examined what would happen to the rate of an enzyme-catalyzed reaction if the concentration of substrate changed. We hypothesized that if the concentration increased‚ then the reaction rate would also increase. To test our question‚ we varied a combination of substrate and buffer‚ totaling 6mL‚ with a constant amount of 2 drops of catalyst. The enzyme catalyst‚ peroxidase‚ increased

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    of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown phosphatase analyzed showed it was an acidic phosphatase. Ph 5 had

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    Enzyme Lab Report

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    Enzymes Ashley Njeru Mrs. Booth Tuesday‚ March 19th 2013 SBI 4U INTRODUCTION: The cellular activity of all organisms is controlled through the use of enzymes. An enzyme is a special type of biological molecule‚ usually a protein‚ which speeds up a chemical reaction; most are soluble in water or a dilute salt solution. There are about 4000 different enzymes in a typical living cell. If even one of these enzymes is missing or

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    Enzyme Lab Report

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    Effects of pH on the Function of Enzymes Rena Mototani Glen Rock High School Advanced Biology 2014-2015 Effects of pH on the Function of Catalase Rena Mototani Problem: How does the pH of a cell affect the function of the enzyme catalase? Introduction In this lab‚ we experimented the effects of pH on the function of the enzyme catalase. Catalase is an enzyme that brings about the reaction by which hydrogen peroxide is decomposed to water and oxygen (Encyclopedia Britannica). The

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    The bonding of an enzyme to its substrate forms an enzyme-substrate complex. The catalytic action of the enzyme converts its substrate into the product or products of the reaction. Each reaction is extremely specific‚ distinguishing between closely related compounds‚ including isomers. For example‚ the enzyme sucrase will only act on sucrose and will not bind to any other disaccharide. The molecular recognition of enzymes is due to the fact that they are proteins‚ which are defined as being macromolecules

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    Enzyme Lab Report

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    specific concentrations; that test the reaction rates of the enzyme catalase over a fixed period of time. The major conclusion was that catalase reacts faster in warm temperatures that are neither freezing nor boiling‚ catalase performs well in lower concentrations than the substrate‚ and catalase prefers neutral pH levels around 7. Introduction Enzymes are proteins that catalyze metabolic reactions vital for the survival and functioning of cells [1]. Without enzymes‚ metabolic processes would occur

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    Enzyme Lab Report

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    This experiment was performed in order to analyze the effects of pHenzyme concentration‚ and temperature on the catalytic rate on enzyme ALP. The initial experiment was done to approximate the optimum pH for the activity of the enzyme. The hypothesis was that alkaline phosphatase activity will be critically affected by pH effects. The hypothesis was valid because the data shows that the pH values for the acidic‚ basic‚ and neutral tubes increased over time‚ absorbing at similar rates. The basic

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    Enzyme Lab Report

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    Biofuel Enzyme Kit Katie Adamson Biochemistry Laboratory‚ BIO124L 1/29/15 Abstract The objective in this lab was to determine the effects different conditions had on the enzyme cellobiase. We examined reaction rates in the presence or absence of an enzyme‚ the effects temperature and pH changes on the enzyme and the effects enzyme concentration and substrate concentration had on the enzyme. As expected results showed us that cellobiase works optimally when conditions are favorable. We see this

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