Abstract The catalysis of enzymes within our bodies is essential for human survival and when this ability is impaired by the presence of Hydrogen ions‚ our cells cannot function properly. This experiment was conducted to determine if the reaction rate changes in response to a variation of acidic‚ neutral‚ and basic solutions. The experimental results indicated that the basic/high pH solution has a faster rate of reaction in the solution. Introduction Enzymes are proteins that catalyze and
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Observing Enzyme Activity Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate. Hypothesis: If the enzyme is in a basic solution‚ then it will react faster because the enzyme (catalase) reacts better in basic solutions. Materials: 10 potato cubes (1 cm3) -Pipet Baking soda solution -50 ml glass beaker Bleach Water Lemon juice Vinegar 5 glass test tubes Drying rack Timer Graduated cylinder Hydrogen peroxide Procedure:
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Finding the Optimal pH of the Enzyme Peroxidase with the Aid of a Spectrophotometer ABSTRACT The peroxidase enzyme was partially purified‚ and the enzyme activity was calculated with the use of a spectrophotometer‚ demonstrating the effect of pH on peroxidase activity. The objective of this exercise was to determine the ideal pH for peroxidase activity. The data concluded that the optimum pH for peroxidase activity is 5‚ and the “standardized” enzyme volume is 2.8mL.
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of an enzyme-controlled reaction. How do these factors affect the chemical structure and properties of the enzyme. Many things can affect the rate of enzyme activity. The temperature of the enzyme‚ the pH of the solution‚ the concentration of the enzyme‚ substrate and the product. Also‚ another affector is the number of competitive and non-competitive inhibitors. As I cannot explain them all‚ I have chosen to explain the effect of temperature and also the effect of inhibitors on enzyme activity
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The aim of the experiment is to test the effect temperature has on the activity of the enzyme rennin. Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC‚ which is the body temperature‚ where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the enzyme will stop working. Introduction:Enzymes are made
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2013 Determining the Mole Ratios in a Chemical Reaction Introduction The purpose of this lab was to determine the mole ratios of the reactants hypochlorite ion (OCI ) and thiosulfate (S O ) when reacted in a chemical reaction. A chemical equation gives the mole ratios of the reactants and products involved in the chemical reaction. When some formulas of the products are not known‚ experimental measurements can be made to determine those ratios. During this reaction‚ hypochlorite
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Thermochemistry Thermochemistry is the study of heat evolved and absorbed during the chemical reactions. The system is the interest of the universe; the surroundings are the rest of the universe in which the exchange of the energy with the system takes place. Both‚ the system and the surroundings make up the universe. Heat flow is the transfer of heat from a warm place to a cooler one. System to surroundings: Surroundings to system:
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that the enzyme concentration will affect the rate of reaction but only up until a certain point because there is a limited supply of substrate. Enzymes are protein molecule that acts as biological catalyst by increasing the rate of reactions without changing the overall process. They are long chain amino acids bound together by peptide bonds. Enzymes are seen in all living cells and controlling the metabolic processes in which they converted nutrients into energy and new cells. Enzymes also help
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experiment on the significant in strength of the enzyme by using three different test tubes and measuring the amount of product they give off. To determine this we are going to test the amount of color absorbance by using a special tool to help us understand our results. We will see how our end results show the effect of the amount of concentration we apply to each test tube. The results would be shown by the support of two graphs. Introduction: Enzymes are highly important because of the significant
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Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab‚ click on the Experiment button to begin the first assignment. This assignment is designed to help you become
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