Use of SDS-PAGE/Western Blot Techniques as Diagnostic Tools for Equine Medical Conditions SDS-PAGE Overview Electrophoresis is commonly used to separate proteins using an applied electrical field. SDS-PAGE separates proteins by molecular weight‚ using sodium dodecyl sulfate (SDS; also known as lauryl sulfate) to denature them and a discontinuous polyacrylamide gel as a sieving matrix. The net charges of folded proteins are not dependent upon molecular weight. Rather‚ charge is determined by
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Impression Creep Behavior of Extruded Mg-Sn Alloy V. Thenambika1‚ S Jayalakshmi1‚ R Arvind Singh2‚ J. Kiruba Nidhi1‚ Manoj Gupta3 1 Department of Mechanical Engineering‚ Bannari Amman institute of Technology‚ Sathyamangalam 2 Department of Aeronautical Engineering‚ Bannari Amman institute of Technology‚ Sathyamangalam 3Department of Mechanical Engineering‚ National University of Singapore (NUS)‚ Singapore Abstract: Mg-Sn alloys contain thermally stable Mg2Sn phase‚ and are proposed as heat-resistant
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What impression do we form of Nick in Chapter One of The Great Gatsby? Nick Caraway is the first character to be introduced in The Great Gatsby‚ whose position of narrator establishes a sense of significance and integrity in novel. Nick writes in Western America‚ having lived briefly in New York during 1922. In the first chapter he begins to address that he encountered difficulties surrounding moral absence during his time in the East‚ adopting a retrospective viewpoint. One of the first impressions
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Topic: Problems related to assessing and classifying/diagnosing 16 to 20 year olds with patterns of impulsivity‚ instability in interpersonal relationships‚ self image and affect. Table of Contents 1. Introduction 2. What is Borderline personality disorder 3. Developmental stages of Borderline Disorder on 16-20 year olds 4. Problems associated with diagnosing and classifying Borderline personality Disorder 5. How its patterns affect
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I have gone through a lot in the past few years. A few years ago‚ I became very depressed. Everything and anything would set me off. My sister‚ Vicki was murdered 24 years ago. One of many triggers has always been her death. Her murder was never solved. My depression was at its lowest when I began having financial problems. I would become so depressed at times that I would cry for hours. I never thought I could crawl out of that hole. At work‚ I was unable to work. At night‚ I was unable to sleep
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DSM-IV Evaluation Sarah Blackerby Abnormal Psychology September 8‚ 2013 University of Phoenix Case Study 1 Josephine is a 47-year-old single woman. The hardest times of the year for Josephine ’s family are holidays and family gatherings; Josephine never seems to enjoy herself. She seldom smiles‚ laughs‚ or reacts to people and events around her. She remains detached and often goes into a room and plays video games or watches television
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DSM-IV Evaluation Case Study 1: Schizoid Personality Disorder “People with schizoid personality disorder persistently avoid and are removed from social relationships and demonstrate little in the way of emotion (APA‚ 2000)” (Comer‚ p. 398‚ 2005). Psychodynamic theorists believe schizoid personality disorder is caused by something that happened through a person’s childhood. They have been unaccepted by their parents or even abused as a young child‚ which causes a person to avoid all relationships
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Case Study Diagnosis PCN 445 December 01‚ 2013 George comes in for treatment. George is an upper middle aged man with support from his wife. George has been having problems for years but either was ashamed of them or did not know where to go. Many people self-diagnose or tell themselves that it is not as bad as they think it is. Or perhaps it will go away in time. Those days turn into months and then years and before you know it you have spent your whole life avoiding certain situations or people
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Case study 1 Josephine is a 47-year-old single woman. The hardest times of the year for Josephine’s family are holidays and family gatherings; Josephine never seems to enjoy herself. She seldom smiles‚ laughs‚ or reacts to people and events around her. She remains detached and often goes into a room and plays video games or watches TV by herself‚ even when the rest of the family is eating. Her family members say that she is rude and do not want her around. Josephine lives by herself. She has
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WHAT IS WRONG WITH THE DSM V Student’s Name: Institution: What Is Wrong With The DSM 5 Since the inception of first DSM in the 1952‚ I believe various psychiatrists have found ways of manipulating the DSM to give a different interpretation of mental disorder just to fit their perception. For instance I noticed DSM I & II observed the patient’s reaction and behavior to a given incident and I found DSM III and IV changed this and ended making people get different perception
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