Hydrolysis of starch for fungal amylase Aspergillus Oryzae and bacterial amylase Bacillus Licheniformis at different temperatures. Maydelis Perez PI 4593042 Florida International University BSC 1010L‚ section UO9‚ March 6‚ 2013 Abstract Enzymes are very specific protein because they contain one active site on their surface that enable the substrate to bind to the enzyme and form the enzyme substrate complex and then release
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precipitate. Starch give positive result in Iodine test as the colour of solution change from yellow to dark blue. Glucose is the simplest carbohydrates that are monosaccharide or simple sugar. Starch consisting of a large number of glucose units joined together by glycosidic linkage and known as polysaccharide‚ that is polymers of sugar. Saliva that contains salivary amylase enzyme‚ which breaks down the glycosidic linkage between glucose that found in starch and hydrolyzes starch into glucose
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Tapioca consists of starch extracted from the cassava root. Cassava was one of the main food sources of Native Americans throughout South America. It is thought that it originated south of the Amazon River in what is now Brazil. It was being cultivated by the people in this region as far back as 8000 BC. By 6000 BC‚ it was being grown in what is now Mexico and in Peru by 2000 BC. Its cultivation would spread throughout the Caribbean well before the arrival of the Europeans. Christopher Columbus
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Molisch Test Color of Junction Glucose Purple Sucrose Purple Starch Purple C. Bial’s Test Color Change Ribose Yellow -> Dirty Green Glucose Yellow -> Dark Yellow D. Seliwanoff’s Test Observation Sucrose 1st (Dark) Glucose 2nd (Medium) Fructose 3rd (Light) E. Benedict’s Test Color Change Glucose light blue -> Orange Fructose light blue -> light olive green Maltose light blue -> yellow brown Sucrose light blue -> blue green Starch light blue -> no change Distilled Water light blue -> no change
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solvency. Many compounds are soluble when exposed to acetone. A Styrofoam and acetone experiment can determine if Styrofoam will dissolve when placed inside a container of acetone. Starch is the major component of flour. Starch exists in tiny granules‚ which swell and break when boiled in water. This releases the starch molecules‚ which then all stick together to make a goopy‚ gluey mess. ii ACKNOWLEDGEMENT First of all I want to thank God for giving me strength for doing this investigatory
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Grace Chung Abstract: Amylase is an important enzyme in the human body as it allows for the consumption of starch by breaking the polysaccharide down into maltose units. All enzymes‚ including amylase‚ function best at a certain optimal pH. Therefore‚ in this experiment‚ the effect of different pHs on the reaction rate of amylase is studied. It was hypothesized that the amylase-starch reaction would proceed fastest at a pH closest to that of the body‚ or 7.0‚ since that is where the enzyme normally
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amylase Bacteria and Fungi with the presence of starch is the purpose of this experiment. Optimal conditions are known as conditions in which organism are able to thrive in‚ where it is able to function at its best. If an organism was to surpass its optimal temperature‚ the denaturing of the active site occurs‚ which means that it changes form‚ which in turn prevents the binding of substrates. For this experiment‚ amylase is an enzyme‚ which starch and glycogen is converted into a simple sugar.
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Bailey Rose The Effects on Enzymes Bailey Rose 10/31/2011 Abstract In this lab exercise‚ the study of enzyme catalase‚ we viewed the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment. Our class performed this experiment by breaking
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Practical 3 Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate Solution | Objective: 1. To show the action of saliva in two carbohydrate solutions. 2. To show the action of hydrochloric acid in two carbohydrate solutions. Apparatus & Equipment’s: Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath‚~37°C Water bath‚~95°C Stop watch
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rate of disappearance of starch molecules from the reaction mixture. Controlled variables: volume and concentration of starch solution; volume and concentration of amylase solution same drop size of iodine solutions; same drop size of reaction mixture taken out for iodine test. Control experiment: reaction mixture without either amylase or starch (this can show that the disappearance of starch is due to the action of amylase on starch). Assumption: 1
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