the following items are needed: Dialysis tubing‚ IKI solution‚ 15% glucose/1% starch solution‚ distilled water‚ balance‚ sucrose solutions (0.2M‚ 0.4M‚ 0.6M‚ 0.8M‚ 1.0M)‚ glucose test tape‚ beakers (multiple)‚ petri dish. In the experiment‚ one will measure diffusion through a selectively permeable membrane (dialysis tubing). A 30 cm by 2.5 cm piece of dialysis tubing that has been soaked in water is acquired and made into a bag by tying one end of the dialysis tubing and opening the other end. Next
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Direction and concentration gradients Bradley Benton ABSTRACT In this experiment‚ we will investigate the effect of solute concentration on osmosis. A semi‐permeable membrane (dialysis tubing) and sucrose will create an osmotic environment similar to that of a cell. Using different concentrations of sucrose (which is unable to cross the membrane) will allow us to examine the net movement of water across the membrane. INTRODUCTION A major determinant of diffusion in a biological system is membrane
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Osmosis SECTION ONE: Methods Lab 1 – Diffusion and Osmosis can be broken down into four parts (A‚ B‚ C‚ and D). Lab 1A – Diffusion In this exercise‚ we tested diffusion through dialysis tubing. This was done through a demo with the teacher‚ rather than individually in groups. First‚ a piece of 2.5cm dialysis tubing was obtained. Then one end was folded and clipped securely so that a solution poured into the bag would not leak out. Next‚ the other side of the bag was opened. For step two‚ a 15%
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experiment‚ the weight of the beaker/dialysis tubing changed and the only logical explanation was that diffusion of water had occurred. Osmosis is the diffusion of water. Depending on which was heavier (the beaker or the dialysis tubing) after the experiment was performed‚ the direction of water diffusion was apparent. If the beaker was heavier‚ then that implies that the water diffused from the dialysis tubing to the beaker. In contrast to this process‚ if the dialysis tubing were heavier after the experiment
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membrane (dialysis tubing)‚ it will be exposed to different environments and concentration gradients. Hypothesis I thought that that dialysis tubing would end up weighing more as there would be less water and more molecules within the tubing so water would move through the semi-permeable using a concentration gradient into the cell. Variables Independent Variable Solution Dependent Variable - Weight of the dialysis tubing Controlled Variable - Length of dialysis tubing
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Diffusion Through Membranes IB Biology 11 Diffusion Through Membranes OBJECTIVES In this experiment‚ you will Use a Conductivity Probe to measure the ionic concentration of various solutions. Study the effect of concentration gradients on the rate of diffusion. Determine if the diffusion rate for a molecule is affected by the presence of a second molecule. BACKGROUND Diffusion is a process that allows ions or molecules
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Purpose To explore transport of materials in three semi-permeable membranes: dialysis tubing‚ eggs‚ and onion cells. Background Information 1. Diffusion is the movement of solutes from high to low concentration. Osmosis is the movement of water from low to high concentration. Both refer to movement in/out of a cell‚ but osmosis refers specifically to water. 2. Isotonic‚ hypertonic‚ and hypotonic all refer to the comparison of concentrations of solutes in verses out of the cell. Isotonic is
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soaked dialysis tubing * 2 dialysis tubing clamps or pieces of twine * 2 clean plastic pipettes * 1 bottle of Lugol’s solution * 2 glucose test strips Procedure: Begin the experiment by placing 4 full pipettes worth of cooked starch in a beaker. Then‚ use a second clean pipette to add 4 full pipettes worth of α-amylase to the beaker and stir to simulate food mixing with saliva in the mouth. Afterwards‚ leaving the mixture in the beaker‚ obtain a piece of soaked dialysis tubing and
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and the iodine caused the color change of the water. 5. Why did a color change occur in the dialysis tubing bag? In the bag there was water‚ sugar (glucose) and starch. When IKI (iodine) comes into contact with the starch the contents in the bag turned blue. This IKI was able to make its way into the dialysis tubing therefore they contact because the IKI molecules are smaller than the holes in the tubing through the process of diffusion. 6. What does the color change in #5 indicate? It indicates
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concentrations of tap water and sucrose‚ the group filled a series of six dialysis tubes each having different concentrations. Of the six dialysis tubes‚ two of them were filled with tap water only‚ two were filled with 20% sucrose‚ and two were filled with 40% sucrose. We then gathered six beakers and filled three of them with water‚ one with 20% sucrose‚ and two with 40% sucrose. Each beaker would then have a dialysis tube added it. Of each beaker filled with just water‚ one received a tube
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