Report August 28‚ 2013 DNA Extraction on Onion (Allium cepa) and Human Cheek Cell Arriza‚ Rolland Merch Buscato‚ Carl G. Butil‚ Conrad G. Leonida‚ Renee Theresa ABSTRACT This activity observes the DNA present in onion (Allium cepa) and human cheek cells by extracting it with the addition of lysis buffer and chilled ethanol. The lysis buffer is prepared from squeezed onion mixed with salt and Pantene Pro-V Shampoo. The buffer degrades the protein enveloping the DNA found in onion and cheek
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Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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DNA profiling is a method of identifying an individual by unique characteristics of their DNA. A specific DNA pattern‚ called a profile‚ is obtained from an individual or a sample of tissue. This allows the comparison of the base sequence of two or more DNA samples to determine whether they are related. DNA profiling has many uses‚ in prevention of economic fraud‚ dietetic work‚ and classifying species‚ identifying bodies‚ forensic science‚ screening for disease‚ and investigating paternity.
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2014/15 Module name and number Molecular Biology & Genetic Engineering: BS941 Assignment title “DNA sequencing: where we are and where it’s going” Student Number 1464986 Word Count 2310 The article focuses on the advances achieved in DNA sequencing by first providing a brief background on DNA‚ and how it was initially sequenced. The paper then takes into consideration four of the major DNA sequencing techniques. These include: Sanger’s Chain Termination Method‚ Pyrosequencing‚ Single Molecule
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The comparison of Cyclops’ from historical contexts to new media In Greek mythology‚ the Cyclops was a member of the giant race‚ every one of them having one eye in the middle of their heads. The Cyclops’ were gigantic monsters who originated from Sicily which consumed human beings. Because of their horrific appearance there were banished to the underworld‚ but later set free from their prison by the Greek gods. In the X-Men series‚ the Cyclops is a character that is portrayed as a superhero
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Compare and Contrast 2 artworks ‘Music In the Tulieries’ And ‘Dance at le Moulin de la Galette’ Music in the Tuileries Garden depicts a fashionable Parisian crowd socialising in the the Tuileries garden as they enjoy the music played by a band‚ Manet has not included the musicians in the painting. The scene is crowded and the composition of people occupies more than half of the canvas. Manet has attempted to capture the hustle-bustle of the modern life of people living during the era. He has
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functions. They both serve as mechanisms for cell division. However‚ quite significantly‚ they are very much different in fact and occur in different situations. Mitosis and meiosis take place in different places. This is one of the first major differences between the two. Mitosis takes place within somatic cells (cells that make up the body)‚ while meiosis takes place within gamete cells (sex cells). As such‚ meiosis produces more gamete cells‚ and mitosis produces somatic cells. Mitosis is the process
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DNA Replication Deoxyribonucleic acid (DNA) contains the genetic instructions for the biological development of a cellular form of life including some viruses. DNA is an antiparallel double helix molecule with sugar-phosphate backbone on the outer side and nitrogen bases in the inner side. The bases are paired specifically‚ also known as complementary pairing‚ Adenine (A) with Thymine (T)‚ and Guanine (G) with Cytosine (C) by two and three hydrogen bonds‚ respectively. DNA is a long polymer
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The difference between plant and animal comes down to cell structure. Animal cells do not have cell walls and have different structures than plant cells. Plant cells only are in rectangular shapes but animal cells are either circular‚ irregular or defined shapes depending on the type of cell. Plants have 3 basic organs; roots‚ stems‚ and leaves. Plants lack a brain and all the organs that animals have to carry out metabolic functions as animals take their carbon from organic substances and plants
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RNA splicing is a process in which certain parts‚ called introns‚ of an RNA molecule are cut out to create a desired RNA strand made out of exons‚ the parts of the RNA molecule that remain and are expressed. As a pre-mRNA molecule is guided through a spliceosome‚ small nuclear ribonucleoprotiens (snRNPs) find the areas where the pre-mRNA’s introns should be cut out. Since the pre-mRNA can be clipped in many different ways through RNA splicing‚ the coding on the pre-mRNA (and future mRNA) strand can
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