Method • Methodology is performed through 3 successive days: First Day: Trypan Blue cell viability assay: Description Trypan blue staining is a simple way to evaluate cell membrane integrity (and thus assume cell proliferation or death) but the method is not sensitive and cannot be adapted for high through put screening. Short 96 well assay: EACH condition should be done in triplicate or more. Steps 1. Trypsinize one T-25 flask and add 5 ml of complete media to trypsinized cells. Centrifuge
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Stainin g What is Cellular Staining? Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that cells
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Abstract This experiment was carried out to perform staining methods on microbes‚ explain the mechanisms of staining‚ namely‚ simple staining‚ and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope were drawn out and labelled. In conclusion‚ different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify
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Micro Biology Lab Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining. Gram Stains is used to identify and classify bacteria‚ it is a differential stain that allows you to classify bacteria as either gram-positive or gram-negative. The foloowing is a breif summerary of the gram staining procedure. Apply the primary stain Apply Mordant Apply decolorizing agent Apply the secondary stain Bacteria that
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Differential Staining Cynthia Alonzo‚ M.S. Version 42-0242-00-01 Lab RepoRt assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information
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STAINING FOR BACTERIAL ISOLATION Submitted by: Cherry Luz L. Rezaga Submitted to: Dr. Tomas J. Fernandez Jr. CRITERIA OF REPORTING 1. Thorough research (20%) 1.1. Number of literature cited more than five (5) 1.2. Completeness of the topic (5) 1.3. Principle discussed (5) 1.4. Literature should be cited in the text (5) 2. Confidence (20%) 2.1. Eye to eye contact (5) 2.2. No reading (5) 2.3. Proper handling of visual aids (5) 2.4. Magnified voice (5) 3. Appropriateness of visual
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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GRAM STAIN THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram
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Gram staining From Wikipedia‚ the free encyclopedia A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923‚ Gram-positive cocci‚ in purple) andEscherichia coli (Escherichia coli ATCC 11775‚ Gram-negative bacilli‚ in red)‚ the most common Gram stain reference bacteria Gram staining (or Gram’s method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). The name comes from its inventor‚ Hans Christian Gram. Gram staining
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