(before inoculation) and before it was closed (after inoculation). (Morello‚ 9th Edition. 2008) EMB (eosin methylene blue) agar was used to determine if the bacteria was a lactose fermenter or a non- lactose fermenter. EMB agar is a selective and differential media. The dyes eosin Y and methylene blue found in the medium inhibit the growth of gram-positive bacteria but not the growth of gram-negatives. Lactose fermenters metabolize the lactose in the media and produce acid byproducts‚ causing a color
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Selective Differential Media Selective Media Population Ingredients Selected (determines what grows ) MSA(p. 105) MAC(p. 117)alphale teathletics @yahoo.co m ( Salt NACL 7.5% makes the medium selective by inhibiting the growth of most bacteria‚ but allowing salt tolerant (Halophile ) staphylo cocci to grow allows staphylococc i to grow b/c they are salt tolerant only disease causing staphylococc i ferment the sugar mannitol
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The different methods of measuring acids and bases in a solution By Starsky Intro to Biology September 26‚ 2011 Lab Partners: Kristen‚ Tania and Betty Introduction When using different methods to measure pH levels there are some tools that can be useful. Some more than others but by putting into action the different methods it may determine which tools will work best and give the best
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Alyssa Caparelli Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane
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Goal(s): To study the effect of concentration on the rate of a reaction‚ and to find the order of the reaction in each reactant and write a rate equation. Background Theory: Chemical reactions occur at different rates that ultimately depend on their concentration‚ the temperature‚ the usage of catalysts‚ the nature of reactants‚ like the surface area of the particles. Concentration‚ for instance‚ increases the number of molecules or particles in a certain volume‚ so collisions will become more
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not exist (Lab Manual 3 pg. 1). They help in many different ways that are useful to the body of living organisms. Enzyme are used to speed up chemical reactions (Lab Manual 3 pg. 1). Through this process‚ they are considered very unique because they are not altered or consumed within the reaction (Lab Manual 3 pg. 1). This is why enzymes are considered biological catalysts. They also do not alter the equilibrium of a chemical reaction nor the amount of free energy that is released (Lab Manual 3 pg
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oxygen‚ carbon dioxide‚ helium‚ and lab gas. We took a Ziplock bag‚ and turned into a non-stretchy balloon‚ and filled with each gas and measured the apparent mass then calculated the actual mass‚ then find a ratio between the mass of oxygen and the other gasses. We tried to keep the pressure about the same each time so our calculations would be more accurate. We found out that the heaviest was carbon dioxide‚ and the lightest was helium. In fact‚ helium and lab gas had a density below 0. Anyways
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Celestial Geography I Emily Richarz PH090-04 Purpose: The purpose of this lab was to introduce us to star charts and how to use them to find stars‚ constellations and other bodies in the sky. Procedure: The instructor gave a brief but thorough lecture and demonstration on how to go about finding the answers on the worksheet. We then filled out the worksheet that cover finding stars and constellations using right ascension and declination in general and on specific dates‚ where constellations
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Lab Report 2 Biological Media Meva Tinsley Georgia State University Lab Report 2 Introduction: Biological Media Microorganisms require nutrients and certain environmental conditions in order to thrive. In the lab‚ we use a Biological Media to aid in growth reproduction. Also referred to as a culture medium‚ a Biological Media is a substance used to support the growth of microorganisms. The two types of media most commonly used in Microbiology are selective media and differential
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Department of biology Faculty of Science and Mathematics Universiti Pendidikan Sultan Idris LABORATORY REPORT SBT 1013: INTRODUCTION TO BIOTECHNOLOGY Semester 4 Sessions 2013/2014 ID NUMBER AND NAME 1. SITI NURFATINI BINTI MOHAMAD ROSLAN (E20131007560) 2.NURHAFIZAH BINTI SAMSUDDIN (E20131007584) 3.MUNIRAH BINTI HARON (E20131007556) 4.NORSYAWANI BINTI SUPIAN (E20131007577) LECTURER : CIK FATIMAH EXPERIMENT NO. : EXPERIMENT NO.1 TITLE : EXTRACTION OF DNA
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