of different concentration of protease. In this experiment‚ a plate of milk agar is needed for comparing the result of each sample. Circular wells were dug on the milk agar plates by the use of cork borer‚ and extracts could be put into the wells by dropper. One must note that milk-agar is white in color due to casein. If protease is present‚ casein will be digested by the enzyme giving a clear zone in the milk-agar plate. The size of the clear zone can help us to determine whether fruits differ
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recorded and the height. * Diffusion in solids -4 agar plates -KMnO4 crystals -methyl orange -refrigeration -ruler The experiment requires 4 plates of agar due to his transparency and the colloid that forms when mixed with water. One pair of plates is labeled 4^0 C and the second pair RT (room temperature). After taking the lids of the first pair ‚using a tooth pick‚ a small crystal of potassium permanganate is placed in the center of the of the agar and the same amount of methyl
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differed between my group’s nutrient agar and MAC agar plates. In courgette‚ there was moderate growth in nutrient agar while it was heavy in Mac agar. In beef broth‚ there was slight growth in nutrient agar while it was negative in MAC agar. In fresh basil‚ growth was heavy in nutrient agar while it was heavy in MAC agar. Where cottage cheese was the ingredient‚ both plates had heavy growth. For walnut‚ growth was slight in nutrient agar while it was negative in MAC agar. Generally‚ the slight difference
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appropriate media using aseptic transfer techniques. The necessity and value of collecting‚ identifying and analyzing is critical for learning how diseases are acquired‚ spread and therefore prevented. Materials Sterile nutrient agar petri dish Wax Pencil Lens paper Sterile blood agar petri dish Igniter Immersion oil Sterile cotton swabs Crystal violet dye Pen Incubator Grams iodine Paper Refrigerator Alcohol acetone Distilled water Safranin dye Inoculating loop Sink Bunsen
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Practical guide to detection and identification of Phytophthora Leaf blight A number of Phytophthora species cause leaf blight. These include: P. infestans on potato and tomato; P. palmivora on a large number of tropical fruit species including rubber‚ durian and macadamia; and P. colocasiae on taro. These blights on leaves are first seen as small flecks but within 3-5 days they expand to produce large lesions. Initially‚ infected tissue is water soaked but becomes necrotic (brown or black) in
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Plant Tissue Culture 151 Chapter 9 Plant Tissue Culture Techniques Lorraine Mineo Department of Biology Lafayette College Easton‚ Pennsylvania 18042 Lorraine Buzas Mineo (B.S.‚ Muhlenberg College; M.A.‚ Duke University) is a lecturer in the Department of Biology‚ Lafayette College‚ and has taught botany since 1978 and supervised the General Biology Laboratories since 1970. Research interests in physiological and forest ecology have culminated in several publications. Other interests include
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CORE BIOLOGY PRACTICALS You will need to know these practicals as the exam board may ask you questions based on them. Below is a summary of each one. Name of practical and independent & dependent variables Effect of caffeine on Daphnia heart rate Independent: caffeine concentration Dependent: heart rate of Daphnia Measuring the content of Vitamin C in fruit juice Independent: fruit juice Dependent: volume of juice required to decolourise 1cm3 of DCPIP The effect of temperature on cell membranes
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Diffusion & Cell Size Lab Background The absorption of nutrients‚ excretion of cellular wastes‚ and the exchange of respiratory gasses are life processes which depend upon the efficient transport of substances into‚ out of‚ and throughout living cells. The process of diffusion can be easily visualized by adding a drop of blue food coloring to a glass of water. Initially‚ the food coloring remains in a small area in the water‚ dying it a dark blue. Over time‚ the molecules of food coloring
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materials used in this study were Duranta leaves‚ Fusarium sp.‚ petri dish for the containment where cultures of Fusarium sp. were grown. Ethanol for extracting the Duranta leaves. Erlenmeyer flask for containment of the concentrations. Potato Dextrose Agar (PDA) served as the food of the fungus. Gloves‚ face masks and lab gown were also needed for laboratory safety rules. Preparation of Different Concentrations and Treatments Fresh Duranta leaves were collected for extraction. Leaves of Duranta
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1 The Effect of Molecular Weight on the Rate of Diffusion of Substances1 Alexander Ken Libranza Group 1 Sec. A – 1L March 6‚ 2012 A scientific paper submitted in partial fulfillment of the requirements in General Biology I laboratory under Prof. Cheryl M. Talde‚ 2nd sem.‚ 2011-2012. 1 2 ABSTRACT The effect of molecular weight on the rate of diffusion was assessed using two tests: the glass tube test and the agar-water gel test. In the glass tube set-up‚ two cotton plugs soaked
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