early days of microbiology in the 19th century‚ culture on agar plates has been a central technique for the study of bacteria. This practical is designed to introduce students to the basic techniques required to manipulate bacteria. Students will gain experience with the streak plate procedure‚ used to isolate pure colonies of bacteria‚ and viable plate count methods. The latter involves serial dilution and spread plating of bacteria on agar plates. Materials required per pair • One 10 ml liquid culture
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Purpose The principle of this experiment is to have a thorough perceptive of: • Culture washed and unwashed lettuce on agar plate. • Culture fresh and opened milk with the same expiration dates. • Explain the significance of food safety. • Illustrate foodborne sicknesses.
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that will be of use to you throughout your biological work. Procedure 1. Heat the test tubes of sterile agar medium in the water bath until the agar melts. 2. Remove the test tubes from the water bath. Let them cool enough to hold in your hand‚ but not so much that the agar becomes solid again. Perform the following transfer as quickly as possible. You must work rapidly so that the liquid agar will not cool and solidify before the transfer is completed. 3. Hold both a test tube
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Abstract: The Preservative effect of brine at different concentrations on lettuce at tropical ambient temperature was examined. The effect of various concentrations of salt solutions on leaf surface and edge browning‚ texture‚ and colour were observed for five days. The experiment was laid out in a Complete Randomized Design (CRD) and each treatment repeated in triplicates including a control. Sensory qualities were examined by six panelists using a 5-point hedonic scale ranking. Results indicated
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The purpose of this lab was to determine the concentration of NaCl or sodium chloride that is isotonic to elodea cells. First off‚ what is osmosis? Osmosis is this huge net of molecules moving through semi-permeable membrane to high concentration to direction where everything becomes balanced. This brings us to what osmolality is. Osmolality is related to osmosis and we know that osmosis has something to do with water so that being said‚ osmolality is number of osmoles of solute per liter in solution
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My results to my findings to my brine shrimp experiment was started on 12-28-15‚ using 4 jars. Each jar contained water‚ salt‚ and brine shrimp eggs. I started by filling each jar with tap water and then adding salt. I added 1 TSP of salt and each jar added ½ TSP more of salt. In my experiment I noticed on the first day‚ 12-28-15‚ in all the jars the brine shrimp eggs were added to the 4 jars. Some observations I noticed were the eggs didn’t sink right away‚ they stayed on the surface for awhile
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[ print page ] Lab Report for HandsOn and Video Observation Labs Question How are cells affected by the movement of water? they expand Hypothesis (What do you think will happen to the egg after the three days? Be sure to give a reason for your hypothesis.) i think the shell of the egg will dissolve and the egg will expand in the liquid. Data and Observations Time (hrs) Observation s Circumference (cm) 0 its a raw hard egg 8cm 24 the shell of the egg has dissolved
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facilities and food products which to be analyzed are swabbed. The swab are diluted in a dilutant such as peptone water or phosphate buffer‚ according to the anticipated amount of contamination and subsequently applied to a growth medium containing agar in a sterile‚ covered plate (David‚ Richard and R. 2004). There are many advantages to the cotton swab method. These include the ease with which any health care
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2 agar plates divided into 4 equal sections were used for this experiment. Each section was labeled with a number from 1-8. 8 Sterile swabs were used‚ 1 for each surface swab. 8 surfaces in my home were then identified that could serve as a fomite and swabbed with a sterile swab that was dipped in distilled water to moisten it. Surface #1 was the garbage disposal in the kitchen sink. It was swabbed and the microbes transferred to the appropriately labeled section marked #1 of the agar plate
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MASINDE MULIRO UNIVERSITY OF SCIENCE AND TECHNOLOGY P.O. BOX 190-50100 KAKAMEGA FACULTY OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES COURSE TILTE: INDUSTRIAL/FIELD ATTACHMENT COURSE CODE: SBL326 NAME: PAMELA K. MUKWEYI REG. No.: BTE/0517/08 DURATION: 9TH MAY – 20TH JULY 2012 SUBMISSION DATE: ATTACHMENT PLACE:CENTRE FOR MICROBIOLOGY RESEARCH- KEMRI Scope/purpose The Industrial Attachment program fulfils part of the requirement in pursuing the degree
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