MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being the
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completed on an agar plate but on one a much larger scale and where techniques can be perfected before the use of agar plates and specimens are used. Because this experiment uses materials that represent those used in the streaking on agar plates the ability to simulate the events that occur when streaking is similar and allows for visualization instantly. In this experiment materials will be gathered that are representative of the tools need to complete the actual experiment of agar plate streaking
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The Effect of Concentration Gradient on Osmosis Abstract: Osmosis is the passive movement of water from an area of higher concentration to an area of lower concentration‚ usually across a membrane (Thorpe 2013). Tonicity is the ability of a solution surrounding a cell to gain or lose water (Reece 2011). There are many factors that affect the rate of osmosis. These include temperature‚ surface area‚ difference in water potential‚ pressure‚ light and dark and most importantly what we will be talking
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Cartesian Diver Essay The Cartesian Diver is named after the French scientist Rene Descartes. This experiment is supposed to show buoyancy‚ density and different forms of matter at work‚ when demonstrated. To create the Cartesian Diver‚ you’ll simply need: • A empty plastic bottle • A plastic eye dropper • Water 1. Fill the bottle with water‚ but be cautious of spills. 2. Very carefully‚ drop the dropper inside the bottle. Then‚ seal the cap on very tightly. 3. Squeeze the bottle‚ but not too
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Discussion The change in the amount of sucrose in the dialysis bag affected the amount of mass each bag loss or obtains. All the tubes contained different amount of sucrose concentrations. The higher molarity concentrations increased the movement of water to balance out the inside of the tube and the beaker. The greater amount of concentration gradient‚ in each tube‚ increased the rate of osmosis. This rate of osmosis is due to the net movement of water from an area of low concentration to
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Reverse Osmosis Application Assignment Osmosis is the dissemination of water atoms through a semi-permeable layer from areas of high to low concentration in the direction that tends to equalize the solute concentration on both sides. In real life osmosis is found in roots of plants retain water from the dirt‚ and kidneys taking water from blood [5]. Reverse osmosis is the removal of solute from water by applying pressure to the water and moving the solution through a semi-permeable membrane. It is
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for antifungal activity of bacterial strain Paenibacillus elgii TS33 25. The nutrient agar medium was prepared‚ sterilized at 121ºC‚ poured into the sterilized peteriplates and allowed to solidify under aseptic conditions. After solidification bacterial strain Paenibacillus elgii TS33 was spot inoculated on nutrient agar medium and incubated at 37ºC for 48 hours. After incubation the molten potato dextrose agar medium containing the spores of test fungus‚ was spread on the same plate and reincubated
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on bacterial growth. By observing the effects of 5 different inhibitors including alcohol‚ bleach‚ soap solution and distilled water‚ it was determined what antiseptic or disinfectant was able to best inhibit this kind of bacterial growth. Nutrient Agar was poured into a Petri dish with four quadrants and then a pipette was used to place bacterial culture on top. Using forceps‚ a filter disc was dipped into each inhibitor and then into a separate quadrant of the Petri dish. The lid of the Petri dish
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INTRODUCTION Total Viable Count is a quantitative idea about the presence of microorganisms such as bacteria‚ yeast and mold in a sample. It counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count‚ like pour plate method‚ spread plate method
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TSI test because there is no pink color for Urea test and no black color on the bottom of the tube in TSI test. Then‚ follow three tests results are all positive. That is deep blue color for citrate test‚ clear zone surrounding growth for skim milk agar
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