TSA (tryptic soy agar) media was placed in a different place to collect cells as well as printed with a thumb print from each member of the group. Incubation is used because each sample needed a certain temperature for the cells to grow. Inspection was used to look at each plate and see how many colonies are found. One TSA plate was placed wherever the student wanted in the building and the other was for each group member to press their thumb print in. The SBA (sheep blood agar) was used for a mouth
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MgSO4 .7H2O‚ 0.2; CaCl2‚ 0.55; NH4Cl‚ 0.4; agar‚ 1.5. A stock solution of the dye was prepared and used for all studies. The sample collected was screened for CV dye decolorizing bacterial strains by inoculating 10 ml of wastewater into flask containing 100 ml of MSM broth. The flasks were incubated at 35°C under shaking conditions (120 rpm). After 48 hr of incubation period‚ 1ml of the culture broth was appropriately diluted and plated on MSM-CV dye amended agar plates. The morphologically distinct bacterial
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cotton with the sterile water then rub gently over areas of the body assigned each group. 1 and 2 – mouth ‚ cheek‚ nose‚ ear‚ nape 3 and 4 – mouth‚ between toes and fingers‚ arm‚ throat 5 – mouth‚ ear‚ between fingers ‚ nose‚ arm 3. Swab the agar plates direct with the cotton swab by simple streaking. 4. Label the plates and incubate for 24 hours. 5. Examine the plates (note the colonies). Experiment #2Normal Flora of the Body | NA | Area Sampled | Color | Whole colony appearance
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ACTIVITY 1: Simulating Dialysis (Simple Diffusion) Answers 1. The molecular weight of urea is approximately 60. Which of the membranes can it pass through? a. 50 MWCO membrane b. 100 MWCO membrane c. 200 MWCO membrane d. Both the 100 and 200 MWCO membranes D. Both the 100 and 200 MWCO membranes 2. True or False: A solution containing glucose will diffuse faster through a 200 MWCO membrane if it is heated. True 3. The molecular weight of carbon C is 12; the molecular weight of hydrogen
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Simple Diffusion 1. The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? According to your results‚ which solute had the highest molecular weight? ______________________________________ Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? __________________________ Using the data from Chart 1‚ explain the relationship between the rate of diffusion and the size of the solute.
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The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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pore protein 3. carrier protein SIMPLE DIFFUSION What is diffusion? Diffusion is the movement of particles from a high density region to a low density region. No energy is needed and no membrane involves in diffusion. The Dynamic Equilibrium Diffusion will continue until the concentration in all region is the same. When this happen‚ we say it has reached the dynamic equilibrium. Factors Affecting the Rate of Diffusion (How fast diffusion happens) Particles that Move Through
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Lab 1: Introduction to Science Exercise 1: Data Interpretation Table 4: Water Quality vs. Fish Population Dissolved Oxygen (ppm) 0 2 4 6 8 10 12 14 16 18 Number of Fish Observed 0 1 3 10 12 13 15 10 12 13 Questions 1. What patterns do you observe based on the information in Table 4? 2. Develop a hypothesis relating to the amount of dissolved oxygen measured in the water sample and the number of fish observed in the body of water. 3. What would your experimental approach be to
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supported by using Fick’s law: rate of diffusion=area x concentration difference/ distance. This formula illustrates that as the concentration difference increases‚ so does the rate of diffusion. Therefore‚ higher concentrated solutions will have a higher rate of diffusion/osmosis. 2a. The purpose of increasing the concentration of sodium chloride was to increase the rate of diffusion. This illustrated that as concentration increased‚ the rate of diffusion also increase. 2b. This experiment
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Review Sheet 1 Results 1. Describe two variables that affect the rate of diffusion. Your answer: Two variables that affect the rate of diffusion are the MWCO membrane and the solute concentration. Increasing the membrane size and solute concentration will also increase the average diffusion rate. Decreasing the membrane size and solute concentration will reduce diffusion rates and can even prevent all diffusion. 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane
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