11M01_ZAO2177_01_SE_CH01.QXD 3/4/11 1:36 AM Page 1 E X E R C I S E 1 Cell Transport Mechanisms and Permeability P R E - L A B Q U I Z 1. Circle the correct term. A passive process‚ diffusion / osmosis is the movement of solute molecules from an area of greater concentration to an area of lesser concentration. 2. A solution surrounding a cell is hypertonic if: a. it contains fewer nonpenetrating solute particles than the interior of the cell. b. it contains
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merely have greatly reduced numbers of “harmless” bacteria. It is often necessary to determine how many live bacteria are actually in a sample‚ especially when measuring growth rates or determining disinfectant effectiveness. This involves MacConkey agar which is a selective and differential
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ACTIVITY ONE- Cell Transport Mechanisms and Permeability: Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. Molecular weight affects the rate of diffusion because the bigger the size of the molecule the longer it will take to diffuse. Membrane size is another variable that affects the rate of diffusion because if the membrane’s pores are small it will take molecules longer to diffuse though it than if the pores were larger. Also‚ membrane thickness
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unknown mixture‚ using a number of laboratory tests. Over the semester‚ multiple tests were carried out to identify between gram-positive and gram-negative bacteria including the Mannitol Salt Agar (MSA) media test‚ which selects only for gram positive bacteria‚ and the use of Eosin Methylene Blue Levine (EMB) Agar media‚ which selects for gram negative bacteria and differentiates between lactose fermenters (paracolons) and non-lactose fermenters (coliforms).These tests along with other selective and/or
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ATCC 8007. The bacterium was activated in Man-Rogosa-Sharpe (MRS) agar medium (Difco‚ BD Diagnostic Systems‚ Maryland‚ USA) consisting of (g L1): peptone casein‚ 30; meat extract‚ 10; yeast extract‚ 6.0; sodium acetate‚ 5.0; ammonium citrate‚ 2.0; glucose‚ 0.2; magnesium sulfate‚ 0.2; manganese sulfate‚ 0.05; dipotassium phosphate‚ 2.0. The pH of the medium was adjusted to 6.5. The master cell bank culture was inoculated into agar plates and incubated for 48 h to produce the working cell culture
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Introduction A computerized simulation experiment will be conducted in order to study the cell transport mechanism via the cell’s selectively permeable membrane and passive processes of simple and facilitated diffusion. The plasma membrane is a gateway which allows nutrients to enter the cell and keep undesirable substances out‚ hence‚ making it selectively permeable. One method of transport is called active transport‚ which uses ATP to transport substances through the membrane. The other is
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4.5 DISCUSSION The bioluminescent bacteria grow well and produce good glowing in the SWC agar media compared to the LA agar media. In LA agar media‚ the production of light was very deem. It also took much time to solidify and the agar media was too soft and forms hole‚ therefore good streaking couldn’t be done. There might be error in the composition of the LA agar media ingredients. However‚ when SWC agar media used‚ there was good growth of bacteria and bright production of light. When comparing
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Activity 1: Stimulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. The two variables that affect the rate of diffusion are: • The composition of the lipid layer‚ this content varies from tissue to tissue. For example kidney tissues have a high cholesterol content which makes them impermeable to water. • The size of the molecule‚ the larger the molecule the slower the rate of diffusion. 2. Why do you think the urea was not able to diffuse
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CHAPTER-1 INTRODUCTION 1 INTRODUCTION The organisation can be defined as “the planned coordination of the activities of a number of people for the achievement of some common‚ explicit purpose or goal‚ through division of labour and function and through a hierarchy of authority and responsibility.” Organisations are not just means used by groups of people to achieve some goals. They present different images like‚ Organisations as machines Organisations as living systems Organisations
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REVIEW SHEET EXERCISE 1 Cell Transport Mechanisms and Permeability NAME: LAB TIME/DATE: Simple Diffusion 1. The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? None of the solutes were able to pass through the 20 MWCO membrane. A possible reason to why none of the of these solutes were able to pass through was because the solutes could have been too big to be transported. According to your results
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