Diffusion and Osmosis Experiment Methods/Materials: 7.1 Experiment: Rate of Diffusion of Solutes In the initial set up of this experiment I had 2 sets of 3 screw-cap test tubes that had each been half-filled with 5% gelatin and 1-mL of the correct dye (either potassium dichromate‚ aniline blue‚ or Janus green) in each of the test tubes. I labeled the 3 test tubes of set 1 with which die they contained and marked them “5 ˚C”. Then with the other set I did the same exact thing‚ except I labeled
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Osmosis and diffusion are sometimes mixed up without much thought‚ but they are very different in many ways. Osmosis is a defined as the movement of water through a semipermeable membrane (). The membrane is fully permeable for the small water molecules‚ but is selectively permeable to any substance that is lager that a normal water molecule. One example might be a glucose and water mixture‚ when the solute is put into one side of a U-shaped tube that has a semipermeable membrane between the solvent
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Making Agar Cushions 1. Start with wearing gloves and swabbing them with 70% ethanol to keep the materials that will be used in the experiment sterile. 2. Wipe the work station with Wescodyne and paper towels. It is important to keep the sterile materials such as slides in petri dish‚ Pasteur pipette in container and forceps covered as much as possible. 3. Place four to five filter papers in the petri dish and drip few drops of water on the strips‚ this will maintain moisture in the agar cushions
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Lab One – Diffusion and Osmosis SECTION ONE: Methods Lab 1 – Diffusion and Osmosis can be broken down into four parts (A‚ B‚ C‚ and D). Lab 1A – Diffusion In this exercise‚ we tested diffusion through dialysis tubing. This was done through a demo with the teacher‚ rather than individually in groups. First‚ a piece of 2.5cm dialysis tubing was obtained. Then one end was folded and clipped securely so that a solution poured into the bag would not leak out. Next‚ the other side of the bag was
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Materials and Methods: Inoculation of the unknown was done on a nutrient slant agar The nutrient agar was then incubated at 27°C for one week. The agar appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain
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Agar and Media Preparation— Agar plates containing King’s B Agar were often used throughout the experiment to support growth of Pseduomonas fluorescens. A recipe was used that included a mixture of 10g Proteose Peptone #3‚ 1.5g Potassium Phosphate Dibasic (K2HPO4)‚ 30ml 50% Glycerol‚ ~965ml water and 20g agar. The mixture‚ post- autoclave‚ was left to cool and 5ml 1M Magnesium Sulfate (MgSO4) was added and created about 40 plates. King’s B Medium was made using the same procedure as the King’s B
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Diffusion is one of several transport phenomena that occur in nature. A distinguishing feature of diffusion is that it results in mixing or mass transport‚ without requiring bulk motion. Thus‚ diffusion should not be confused with convection‚ or advections‚ which are other transport mechanisms that utilize bulk motion to move particles from one place to another. In Latin‚ "diffundere" means "to spread out". There are two ways to introduce the notion of diffusion: either a phenomenological approach
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Diffusion is the process by which a particular phenomenon --- such as a contagious disease‚ a technological innovation or even an idea --- is spread from person to person over a period of space and time. One method of diffusion is known as expansion diffusion‚ which is broken down into three distinct types. Expansion Diffusion * Strictly defined‚ expansion diffusion is the process of spreading something from one place to another in an ever-expanding "snowballing" process. Expansion diffusion
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Answers to Questions Activity 1: Simulating Dialysis (Simple Diffusion) (pp. 2–4) 9. All solutes except albumin are able to diffuse into the right beaker. Using distilled water in the right beaker and either the 100 MWCO or 200 MWCO membrane will remove urea from the left beaker and leave albumin If the left beaker contains NaCl‚ urea‚ and albumin‚ you can selectively remove urea by dispensing a concentration of NaCl into the right beaker equivalent to that in the left beaker and by using
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Marie Gorjon Mrs. B Anatomy & Physiology 168 March 14‚ 2014 Diffusion across a Selectively Permeable membrane Introduction: Diffusion is movement of molecules from one area of concentration to another. This process is vital for the life functions of cells. Cells have selectively permeable membranes that allow only certain solutions to pass through them. Osmosis is a special kind of diffusion that allows water to go through semi-permeable membranes of high water potential to a region of lower
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