The unusual members of NFGNB represent a challenge for the routine clinical microbiological laboratories‚ owing to their complex identification along with their unpredictable antimicrobial susceptibility patterns 14. The isolation rate of NFGNB (18.7%) recorded in our study comes in agreement with a previous Indian study carried out in a tertiary care hospital‚ where it was 16.18% 15. Whereas‚ it comes higher the result recorded in another Indian tertiary care hospital where it was 9.32% 16. The
Premium Patient Health care Medicine
aureus were maintained on Tryptic Soy agar (TSA)‚ following incubation at 37 oC for 24 h. Inocula were obtained from overnight fresh cultures adjusted to approximately log 108 CFU/ml‚ a turbidity equivalent to a 0.5 McFarland standard (Kroning et al.‚ 2016). The inocula were spread on the surface of Mueller-Hinton agar (MHA) plates. The disk diffusion test‚ recommended by the Clinical and Laboratory Standards Institute (CLSI‚ 2015)‚ was employed
Premium DNA Gene Protein
effectiveness of an antimicrobial agent against bacteria grown in culture. This is called the Kirby-Bauer disk-diffusion method‚ and here is how it works. The bacteria of interest are swabbed uniformly across a culture plate. Then a filter-paper disk‚ impregnated with the compound to be tested‚ is placed on the surface of the agar. The compound diffuses out from the filter paper into the agar. The concentration of the compound will be higher next to the disk‚ and will decrease gradually as distance
Premium Antiseptic Bacteria Microbiology
fungus grows. Therefore optimum temperature will increase growth. The diffusion rate of these dead organic matters is affected by temperature. According to Kinetic theory higher temperatures increase the energy and therefore the movement of the molecules‚ increasing the rate of diffusion‚ therefore increasing growth. Lower temperatures decrease the energy of the molecules‚ thus decreasing the rate of diffusion. The optimum diffusion rate varies with different fungi’s. The growth of Aspergillus oryzae
Free Agar plate Petri dish Fungus
recorded and the height. * Diffusion in solids -4 agar plates -KMnO4 crystals -methyl orange -refrigeration -ruler The experiment requires 4 plates of agar due to his transparency and the colloid that forms when mixed with water. One pair of plates is labeled 4^0 C and the second pair RT (room temperature). After taking the lids of the first pair ‚using a tooth pick‚ a small crystal of potassium permanganate is placed in the center of the of the agar and the same amount of methyl
Premium Diffusion Petri dish Laboratory glassware
Antibacterial activity of allopathic medicine versus phytomedicine against Enterococcus faecalis: An in-vitro study. Abstract Background: A successful endodontic treatment depends on appropriate instrumentation‚ irrigation‚ decontamination and obturation. Decontamination of root canal plays a crucial role in the success of endodontic treatment. Therefore‚ a suitable medicament is essential which can effectively eliminate bacterial contamination. Aim and objectives: To investigate and compare the
Premium Pharmacology Bacteria Antibiotic resistance
have a greater surface area than spheres of the same volume. In Part B‚ the relationship between diffusion and surface area to volume ratio was investigated. Three agar-phenolphthalein-sodium hydroxide cubes of different sizes were exposed to a sulfuric acid solution. It was found that the cube with the smallest volume had the highest rate of diffusion. It was concluded that since life-preserving diffusion is more efficient for small cells‚ size influences growth and
Premium Volume Chemistry Water
Petri dish- This is used to transfer the agar cubes as you should put the cubes in there as some of the chemicals can be dangerous Safety goggles- These are essential to keep your eyes protected from harmful chemicals White tile- To help to see the colour change Stop clock- To time how long it takes for the cube to change colour. Method 1-Stop the clock and quickly but carefully place one of the agar cubes into the specimen tube containing 3.7g/100cm3 of hydrochloric acid
Premium Water Dehydration Potassium
The agar diffusion study of steam distilled ethanolic extract of aerial parts of the plant shows strong inhibition of plant pathogens‚ Rhizoctonia sp. Botryosphaeria rhodina and two strains of Alternaria sp. (Alternaria sp. (A1) and Alternaria sp. (A2) - tomato wilt fungi) which was mainly due to presence of fungitoxic phenolic compound eugenol. When this eugenol was further subjected to agar diffusion assay it shows strong efficacy against Alternaria
Premium Bacteria Immune system Skin
will become hypotonic and swell up as the time increased. “Diffusion is a passive process in which the random mixing of particles in a solution occurs because of the particle ’s kinetic energy “(Allen C. 2006)‚ and is subjected to detect the presence of each of the substances given in the biochemical testing‚ the increase in temperature‚ and different reagent used are some aspect that could cause the difference of diffusion in certain solution. It was clear that the protocols Glucose‚ Albumin
Premium Cell membrane Diffusion Molecular diffusion