Susceptibility test (disc diffusion technique). Each group will inoculate his/her own plate of Mueller-Hinton agar with an assigned culture. To that inoculated plate‚ you will then aseptically add sterile filter paper discs (using a disc dispenser)‚ which contain a known concentration of antibiotics. As soon as the antibiotic discs touch the agar‚ the antibiotic will begin to diffuse into the surrounding agar. During incubation the bacteria you inoculated onto the agar
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performed by agar disc diffusion method. Muella Hinton agar was prepared and autoclaved at a specified temperature and pressure. The medium was then cooled and 20mL of the medium was poured into a sterile petri dish and allowed to solidify. A sterile wire loop was used to transfer a colony of test organisms into a test tube with 2mL peptide water. A swab stick was dipped into the test tube containing the organisms and it was used to seed the organism on the solidified Muella Hinton agar. The prepared
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incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion is the movement of particles from an area of higher concentration to a lower concentration until they reach Equilibrium. (Hickman‚ 2008).Osmosis happens when water diffuses across the membrane from the region of lower solute concentration (higher
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90celcius which will convert the Chitin into Chitosan upon dissolving. The third phase will take place over 3 days. The nutrient broth and nutrient agar will be prepared first. On the 2nd day‚ the bacteria broth will be prepared prior to an overnight culture on the same day using E-Coli and M.Luteus. On the 3rd day‚ the Anti-bacterial well diffusion test will be carried out. Finally‚ we will
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sterilizing it with 70% ethanol. Then trypticase soy agar (TSA) was poured into six groups of 60 Petri dishes (See Appendix 1). The dishes were labeled based on the antibiotic used and were left to dry and solidify at room temperature. After an hour‚ the dishes were placed in a refrigerator at three degrees Celsius. If there was visible condensation on the agar plate‚ they were flipped to avoid the contact of heat that drove the moisture out of the agar dishes. The contents of the broth culture of E.
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identified the need to determine a given bacterium’s susceptibility or resistance to a given drug which prompted W. M. M. Kirby and A. W. Bauer to develop a single disk method for susceptibility testing. This experiment used the Kirby-Bauer disk diffusion test to measure the degree to which Penicillin‚ Streptomycin‚ Ampicillin‚ and Chloramphenicol inhibited the growth of the bacterium‚ Proteus vulgaris. The measured zone of resistance for each antibiotic was compared against antibiotic performance
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test antimicrobial activity against disease causing bacteria Staphylococcuaureus. Extracts of varying concentrations of Azadirachtaindica fruit pulp and leaf extract were prepared using Phosphate Buffer and tested against test organisms using agar diffusion method. Oxfloxacinof same varying concentrations were used to compare the effect of antimicrobial activity of fruit pulp and leaf extract. Keywords: Azadirachtaindica‚ Antimicrobial activity‚ Staphylococcus aureus. I. INTRODUCTION Azadirachtaindica
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Research II Summarized Project Proposal Title: The Efficacy of Lubi-lubi (Atropa belladonna) Fruit Extract as Anti-neoplastic Agent against Human Papilloma Virus Related Cervical Neoplasms Problem: Is the Lubi-lubi extract a potent anti-neoplastic agent against HPV related cervical neoplasms? Specific Problems: 1. What stage in the maturity of the deadly nightshade fruit is most efficient as an anti-neoplastic agent? 2. What concentration of the extract is most efficient
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points Describe the Kirby Bauer Test? Make sure you describe all the key elements. 1. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 2. Place antibiotic disks evenly spaced on the inoculated agar plates and incubate at 37C for 24-48 hours. 3. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 4. Using sterile technique place disk in each of the solutions:
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which can fight of antibiotics allowing the bacteria to become antibiotic resistant. In this lab report‚ we discover the most effective disinfectant that would work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly with my results but Lysol was only 4mm more sustainable then Windex. Lysol
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