group’s hypothesis was that “cells are small because small cells have a larger surface area to volume ratio”‚ which is important because a larger surface area allows for more molecules to diffuse at one time. The group created cells made out of blocks of agar-phenolphthalein. This allowed a simple indicator of pH‚ since phenolphthalein turns magenta when it comes into contact with a base.
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potential. Methods Antibacterial activity of aqueous and organic seed extracts was assessed using agar diffusion assay‚ minimum inhibitory concentration and viable cell count studies; and their antibacterial effect was compared with some standard antibiotics. The presence of major phytoconstituents was detected qualitatively and quantitatively. The isolated phytoconstituents were subjected to disc diffusion assay to ascertain their antibacterial effect. Results Hot water and acetone seed extracts
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this carbon and it is a key enzyme in the bio refinery process of producing green chemicals (Ponnambalam et al.‚ 2011). In screening for the cellulolytic fungi‚ several qualitative display of cellulolytic such congo red clearing zone assay‚ gel diffusion assay and dyed congo red filter paper clearing zone assay can be used. In this screening‚ congo red clearing zone assay is performed on 9 unknown fungi isolates on CMC media. Cellulose degradation and its subsequent utilizations are important for
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investigated compounds were dissolved in DMSO at 10 and 20 mg ml-1‚separately [19‚21‚25‚27‚34]. Nutrient agar was prepared‚ then sterilized in an autoclave and poured in sterile Petri plates. After cooling of nutrient ager in Petri plates‚ the studied organisms were grown on agar. After that‚ the sterili Paper discs of Whatman saturated with solution of investigated compounds were placed in the agar by working holes using a sterile crook borer. These Petri dishes were incubated for 24 h at 37oC [25]
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of bacteria in a given cultured sample is too great to be counted directly. The samples will be serially diluted by a factor of 10 in sterile saline‚ to be repeated 7 times. A 10 microliter aliquot from each dilution will be placed on a 5% blood agar plate or a MacConkey plate‚ depending on the type of organisms plated. These plates are selected because of the ease of differentiation between organisms. The original vial and all diluted aliquots will then be refrigerated to restrict growth until
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2 were Pseudomonas aeruginosa. One of each was used to confirm. Finally only MTCC cultures were used to prepare inoculum. Inoculums Preparation Primary isolation was initiated by nutrient agar plating and subsequently transferred to selective media like Pseudomonas Isolation Agar‚ MacConkey Agar and EMB agar for better isolation. Using sterile inoculation loop colonies of the test organism are transferred to 5ml of sterile nutrient broth and incubated at 37 0C overnight for 24hrs. Then this bacterial
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constituent. It refers to the component of the plant responsible for the exhibitory activity of the drug.1 Agar. It is gelatinous colloidal extractive of a red alga (as of the genera Gelidium‚ Gracilaria‚ and Eucheuma) used especially in culture media or as a gelling and stabilizing agent in foods.2 Agar-well diffusion method.3 It is a method of bioassay wherein wells are cut in seeded agar and the sample is then introduced directly into the wells. After incubation‚ the diameter of the clear zone
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against (Staphylococcus aureus‚ Staphylococcus epidermidis‚ Escherichia coli‚ Pseudomonas aerugenosa‚ Klebsiella pneumoniae‚ Proteus mirabilis and yeast Candida albicans). Two methods were employed for propolis activity evaluation; firstly by agar well diffusion method using 40µl of propolis extracts and ethanol 70% as the solvent control per well against tested bacteria and fungus and more commonly used antibiotics were used in comparison to propolis samples. The second method was employed by measuring
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active transport? What is passive transport? How is osmosis related to diffusion? How can we demonstrate active transport? How can we demonstrate Brownian movement? How can we demonstrate diffusion (2 ways)? How can we demonstrate osmosis (3 ways)? In terms of relationships between substances‚ how can we define “hypertonic”‚ “isotonic”‚ and “hypotonic”? What is the relationship between the size of a molecule and its rate of diffusion? ____________________________________________________________________________
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EFFECT OF USED OIL IN Trichoderma harzianum A Thesis Presented to the Faculty‚ Special Science Class Iloilo National High School La Paz‚ Iloilo City In the Partial Fulfillment in the subject Research III Sheree Joie Montomo Polonan January 2013 Chapter 1 Introduction of the Study Chapter 1 is divided into five parts: (1) Background of the Study‚ (2) Statement of the Problem and Hypothesis‚ (3) Significance of the Study‚ (4) Definition of Terms
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