UNIVERSITY OF DAR –ES-SALAAM COLLEGE OF NATURAL AND APPLIED SCIENCES DEPARTMENT OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY MC206: FOOD MICROBIOLOGY PRACTICALS PRACTICAL 1 MICROORGANISMS IN THE ENVIRONMENT GROUP #:1 NAME: DUSENGEMUNGU Léonce REG #: 2011-04-07086 COURSE INSTRUCTOR: Dr Mugassa S.T Rubindamayugi
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source. Simmon’s citrate agar will be prepared by using Oxoid well-prepared Simmon’s citrate agar powder. The Simmon’s citrate agar was prepared by using Oxoid well-prepared Simmon’s citrate agar powder. The preparation was began by suspending 5.75g of the powder in 250 ml of distilled water and mixed thoroughly. The following shows the amount of each composition in 1 litre of Simmon’s citrate agar solution prepared using Simmon’s citrate agar powder. This Simmon’s citrate agar solution was then heated
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patients such as those suffering from cystic fibrosis or immunocompromised individuals. MIC methods are varies such as disc tests that give qualitative result‚ dilution tests which testing the ability of microorganisms to produce visible growth on several agar plates. It containing dilutions of the culture and antimicrobial agent.
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were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables‚ charts and drawings herein and reflect the results of microscopic observations as well as the differential tests results on various agars and broth cultures. Although all tests were not conclusive‚ the unknown organism labeled Unknown #11 was found to be a member of the family Enterobacteriacea and Genus Serratia marcescens. INTRODUCTION The field of Microbiology is the
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known as "red seaweed." The specific chemical that we are interested in is agar‚ which appears in red seaweed in abundance. When you hear Cereplast and other companies talk about developing bioplastic made from seaweed‚ they really mean that they will be using the chemical agar‚ which is extracted from the seaweed. Fortunately (or unfortunately?) this project won’t have you traipsing out to the ocean to collect seaweed. Agar is used as a food additive in confectionaries‚ desserts‚ beverages‚ icecream
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a given sample. It enumerates the number of actual live‚ viable cells in the sample that form colonies on a suitable agar medium. As the optimum medium and conditions varies for one sample to another‚ the colony count methods provide an estimate of the number of viable cells according to the medium employed‚ time and temperature of incubation. Each colony that appears on the agar plate arising either from a clump of cells or from a single cell is referred as a colony forming unit (CFU). The sample
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Chapter 1 The Problem Introduction Today’s technological innovation and medical advances gave birth to formulation of synthetic drugs used to treat respiratory infections‚ urinary tract infections‚ diarrhea‚ arthritis and other diseases. These pharmaceuticals are costly and to a point‚ inaccessible to many Filipinos. These may also pose adverse effects like nausea‚ dyspnea‚ and diarrhea. Researchers are challenged to produce natural medicines‚ which could be lower in cost and more synergistic
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facilitates the handling of large numbers of bacterial clones for classification on a variety of media. METHODS Replica plating. A frequent chore in bacteriological work is the transfer of isolates from one substrate to other selective or indicator agar media. In place of an inoculating needle‚ one might imagine a device consisting of many needle tips in fixed array‚ so that one operation would substitute for repeated transfers with a single needle. The requirements of this design are met by pile
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stain. * Observe the slides under microscope and record results. BIOCHEMICAL TESTS SUSCEPTIVITY TESTING Materials * MHA agar * Organism grown in Broth * Swabbing stick * Principle Procedure TSI (TRIPLE SUGAR IRON TEST) Materials * TSI agar * Straight wire loop * Cultured colony of organism * Purity plate Principle TSI agar detects fermentation of glucose 0.1%‚ lactose 1% and sucrose 1%‚ production of H2S‚ and gas formation. The medium contains ferric
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bacteria Bacillus subtilis did not grow well to see if there was a clearing. After doing some research‚ it was shown that B. subtilis is lipase positive so there should have also been some clear sones like the Staphylococcus aureus (Watson). The DNAse agar could not have been analyzed because HCl was not available to precipitate the DNA to see if the bacterium had the enzyme DNase. If the test could have been analyzed‚ Staphylococcus aureus would be positive for DNase and E. coli would have been negative
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